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AIM To optimize the supercritical CO2 extraction of sauchinone and to evaluate the in vitro antitumor activity of Saururi Herba supercritical extract.METHODS With extraction pressure,extraction temperature,extraction time,entrainer (ethanol) concentration and entrainer amount as influencing factors,together with extraction rate of sauchinone as an evaluation index,orthogonal test was used for optimizing the extraction.Then MTT was applied to determining the extract's inhibitory effect on human multidrug-resistant hepatocellular carcinoma cell line (7721/Adm).RESULTS The optimal conditions were determined to be 30 MPa for extraction pressure,50 ℃ for extraction temperature,2 h for extraction time,95％ for ethanol concentration,and one time for ethanol amount,the average extraction rate of sauchinone was 0.173％.The obtained extract significantly inhibited the proliferation of 7721/Adm cells (IC50 =50.08 μg/mL),demonstrating a stronger activity than that of ethanol extract (ICs0 =150.59 μg/mL).CONCLUSION This stable and feasible method is appropriate for sauchinone extraction,and the supercritical CO2 extract from Saururi Herba shows a strong in vitro antitumor activity.
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OBJECTIVE:To analyze the differences of inorganic elements in different parts of Saururus chinensis.METHODS:The samples were treated with microwave digestion.The contents of 25 inorganic elements were determined by inductively coupled plasma mass spectrometry (ICP-MS) method:RF power of 1 200 W,cooling gas flow of 14 L/min,auxiliary gas flow of 1.0 L/ min,atomizing air flow of 0.9 L/min,He as collision gas with flow rate of 4.8 mL/min,sampling cone aperture of 1.1 mm,skimmer cone aperture of 0.9 mm,super skimmer cone aperture of 1.0 mm,integral time of 1 s,delay time of 40 s,single hop as scanning mode,repeated for 3 times.The data of principal component analysis and partial least squares discriminant analysis were analyzed with the SIMCA-P 13.0 statistical software.RESULTS:The linear ranges of lithium (Li),beryllium (Be),boron (B),magnesium (Mg),aluminum (Al),titanium (Ti),vanadium (V),chromium (Cr),manganese (Mn),iron (Fe),cobalt (Co),nickel (Ni),copper (Cu),zinc (Zn),gallium (Ga),arsenic (As),cobalt (Sr),cadmium (Cd),tin (Sn),antimony (Sb),barium (Ba),mercury (Hg),thallium (Ti),lead (Pb) and bismuth (Bi) were 0.05-5.0 μg/L(r=0.999 9),0.05-5.0 μg/L(r=0.999 8),0.1-10.0 μg/L(r=0.999 5),1.0-100.0 μg/L(r=0.999 5),1.00-100.0 μg/L(r=0.999 3),0.10-10.0 μg/L(r=0.999 9),0.05-5.0 μg/L (r=0.999 7),0.05-5.0 μg/L(r=0.999 5),0.10-10.0 μg/L(r=0.999 8),1.00-100.0 μg/L(r=0.999 4),0.05-5.0 μg/L(r=0.999 9),0.05-5.0 μg/L(r=0.999 7),0.05-5.0 μg/L(r=0.999 5),0.05-5.0 μg/L(r=0.999 8),0.05-5.0 μg/L(r=0.999 6),0.05-5.0 μg/L(r=0.999 8),0.05-5.0 μg/L(r=0.999 9),0.05-5.0 μg/L(r=0.999 3),0.05-5.0 μg/L(r=0.999 4),0.05-5.0 μg/L(r=0.999 8),0.1-10.0 μg/L(r=0.999 6),0.05-5.0 μg/L(r=0.999 6),0.05-5.0 μg/L(r=0.999 6),0.05-5.0 μg/L(r=0.999 7),0.05-5.0 μg/L(r=0.999 4),respectively.The detection limits were no higher than 13.2 ng/L.RSDs of precision,stability and reproducibility tests were all lower than 5.12％.The recoveries ranged 90.18％-123.01％ (RSD were 2.05％-5.79％,n=6).The contents of Sb,Sn,Mn,Cd,Ti,Ba,Co,As,Be,Ga,Ni were higher in aerial part than in rhizome part,while the content of Cr was higher in rhizome part than in aerial part.CONCLUSIONS:Mg,Fe and A1 are abundant in aerial part and rhizome part of S.chinensis.The contents of inorganic elements are significantly different in different parts of S.chinensis.
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Objective To investigate the systemic effects of Helicobacter pylori (Hp) infection by comparing differential counts of leukocytes in peripheral blood and C-reactive protein (CRP) levels before and after eradication of Hp. Method A total of 158 Hp-positive patients underwent eradication by standard one-week triple therapy were divided into Hp eradicated group(n = 108) and Hp non-eradicated group(n = 50),the populations of peripheral blood leukocytes and CRP levels before and 0 (just after therapy),1 and 12 months after eradication were retrospectively analysed. Results In the eradicated group,blood leukecytes,neutrophils and monocytes decreased significantly after eradication,and the CRP levels were significantly lower than the levels before treatment (P < 0.05 ),but there was no significant change in eosinophils,basophils and lymphecytes (P > 0.05).In the non-eradicated group,there was no significant change in any studied pa-rameter (P > 0.05). Conclusion These findings suggest that Hp infection increase blood leukocytes,neu-trophil and monocyte counts in the peripheral blood and CRP levels,which indicates a significant role of Hp infection in systemic disorders.