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OBJECTIVE: To measure the implicit attitude of nurses towards doctors and analyze its influencing factors. METHODS: A total of 356 nurses were selected as study subjects by convenient sampling method. Their implicit attitude towards doctors was measured by Single Category Implicit Association Test. The related influencing factors were analyzed. RESULTS: The response time of nurses on incompatible tasks was higher than that on compatible tasks [(0.87±0.19) vs(0.76±0.15) s,P<0.01]. The median(M) of D value was 0.34. D value of junior college group was higher than that of undergraduate and above group(M: 0.40 vs 0.27, P<0.01). D value in the nurse group was higher than that in the nurse practitioner group and nurse-in-charge and above group(M: 0.43 vs 0.33, 0.43 vs 0.23, P<0.05). D value of the informal staff group was higher than that of the formal staff group(M: 0.35 vs 0.19, P<0.05). The results of ordered multiple Logistic regression analysis showed that establishment was an independent influencing factor of the implicit attitude of nurses towards doctors(P<0.05), after excluding the influence of confounding factors. CONCLUSION: The implicit attitude of nurses towards doctors is positive and mainly affected by the establishment.
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Objective:To explore effect of high glucose on expression of osteoprotegerin (OPG) and receptor activator of NF-毷B ligand (RANKL) in rat aortic vascular smooth muscle cells. Methods: SD rats were intraperitoneally injected with streptozotocin, OPG and RANKL expression in rat thoracic aortas were detected by immunohistochemical staining. In cultured vascular smooth muscle cells (VSMCs) (A7r5), qRT-PCR and Western blot analysis were used to examine the mRNA and protein levels of OPG and RANKL.Results: Our results demonstrated that OPG expression was increased in hyperglycemic rat aortic VSMCs, while RANKL expression was decreased. Besides,in vitroexperiments high glucose induced OPG expression, but depressed RANKL expression by dose- and time-dependent manner in cultured A7r5.Conclusions: Our findings suggested that high glucose could promote the expression of OPG, and inhibit the expression of RANKL in VSMCs, which may be partly be the molecular mechanism of diabetic vascular calcification.
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Objective To explore the effects of PBL teaching method on classroom atmosphere of basic nursing course.Methods Two classes of nursing sophomore were enrolled and were divided two groups,60 as PBL group and other 60 as traditional group.Students in two groups were evaluated by questionnaire of classroom atmosphere.Data were statistically analyzed through t-test,P＜0.05 standing for statistical significances.Results There were statistical differences in classroom atmosphere between two groups (P ＜ 0.05) ; class democracy,elicitation in teaching and student cooperation in PBL group were significantly better than those of traditional mode (P ＜ 0.05).There was no significant difference in learning autonomy between the two groups (P ＞0.05).Conclusions PBL teaching is conductive to stimulating students' learning potentials,culturing their cooperative learning,raising the efficiency of classroom learning and improving the classroom atmosphere.But learning autonomy of students needs to be further improved.