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Objective To investigate the protective effect of umbilical cord mesenchymal stem cells (UCMSCs) on traumatic brain injury (TBI) in rats.Methods Thirty healthy Sprague-Dawley rats (10 rats for each group) were randomly divided into normal control group (normal),model group (injection of saline after TBI) and UCMSCs transplantation group (injection of UCMSCs after TBI).The rats in experimental groups were sacrificed on the 10th day after UCMSCs transplantation.The percentage of UCMSCs in brain tissue was detected by flow cytometry.The pathological changes of brain tissue were observed by hematoxylin-eosin (HE) staining method.The expressions of vascular endothelial growth factor (VEGF),glial fibrillary acidic protein (GFAP) and brain-derived neurotrophic factor (BDNF) in brain tissue were measured by immunohistochemistry and immunofluorescence double staining.The neurological deficit was evaluated by neurological deficit degree.Results The percentage of CD90,CD73 and CD105 cells in the UCMSCs transplantation group was significandtly higher than that in the model group (0.4％ vs 0.1％,P＜0.05).The results of HE staining showed that the brain injury of the transplanted group was alleviated compared with the model group (P＜0.05).The VEGF of the brain tissue in injury area in the UCMSCs transplantation group was higher than that in the model group (P＜0.05).The number of GFAP and BDNF positive cells in the UCMSCs transplantation group was higher than that in the model group (P＜0.05),and the neurological deficit score was also higher than that in the model group (P＜0.05).Conclusions UCMSCs transplantation for the treatment of TBI rats can effectively reduce the vascular damage in the injury area and promote nerve recovery.
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Objective To investigate the effects of human umbilical cord mesenchymal stem cells (UC-MSCs ) on vascular endothelial growth factor ( VEGF ) and monocyte chemoattractant protein-1 ( MCP-1 ) of acute myocardial ischemia-reperfusion (AMI-R) injury in rats. Methods 24 Sprague-Dawley rats were randomly divided into sham group, AMI-R group and UCMSCs treatment groups on average. The rats were sacrificed on the 10th day after UCMSCs transplantation, and the myocardial tissues below the ligature were taken. The mRNA and protein expressions of MCP-1 of the tissue were detected by RT-PCR and Western Blot respectively, and the expression of VEGF protein was detected by immunohistochemistry. Results The relative expression levels of MCP-1 mRNA and the protein in UCMSCs group were significantly lower than those in sham group and AMI-R group (all P<0.05). The expression of VEGF protein in UCMSCs group was significantly higher than that in sham group and AMI-R group, the differences were statistically significant(all P<0.05). Conclusion UCMSCs transplantation can promote the angiogenesis and decrease the inflammation reaction in the treatment of acute myocardial ischemia-reperfusion injury.
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In recent years,the medical imaging technology has developed rapidly.New imaging methods are unceasingly emerging,and the existed imaging modes have been continuously improved.The trends of medical imaging technology have developed from two-dimension imaging to three-dimension imaging,from localized imaging to whole-body imaging,from static imaging to dynamic imaging and from structure to function.This thesis introduces the new imaging methods,such as Molecular Imaging,Near Infrared Imaging and so on.
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Objective The image segmentation of low-density gene chips based on genetic algorithm, which can implement the function of region identification, is achieved. Methods After image denoising by wavelet analysis, image segmentation is accomplished by genetic algorithm. Results The method can detect the region of sampling point more precisely. It can also effectively separate the valuable weak signal points and background or noise. Conclusion The method can accomplish the function of image segmentation of low-density gene chips, which can provide relative accurate data information for future image analysis
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Objective To design an image pre-processing system of low-density gene chips based on MATLAB, which can process the colored images by cy3 and cy5 fluorescence staining of low-density chips obtained by the array scanning system. It can filter out noise, enhance the contrast gradient of image, improve the quality of image, and implement the functions of image segmentation, edge detection and region identification. Methods The median filter method of the wavelet was used to implement the function of image denoising and improve the image quality. Edge detection was accomplished by wavelet, combining with edge operators. Image segmentation was developed by genetic algorithms. Results It could reduce the effect of spot, noise and other factors, improve the quality of image, and detect the periphery of image better and the region of sampling point more precisely. It can also effectively separate the valuable weak signal points and background or noise with the system. Conclusion The system can accomplish the functions of image pre-processing of low-density gene chips, and the adopted methods are feasible. It can provide relative accurate data information for future analysis.
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Diastolic transmitral blood flow was measured by Doppler echocardiography in 66 patients with hypertension and 33 normal persons. The results showed that isovolumic ventricular relaxation time and peak. A velocity increased and E/A ratio decreased significantly in hypertensive patients without left ventricular (LV) hypertrophy. Patients with LV hypertrophy had not only significant change of these parameters, but also significant decrease of peak E velocity and deceleration of E velocity. Left atria was larger in hypertensive patients, especially in those with LV hypertrophy. It is suggested that LV diastolic dysfunction in patients with hypertension may occur before the development of LV hypertrophy, and LV hypertrophy may worsen the diastolic dysfunction.
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Plasma concentrations of ?-endorphin (?-EP), leucine enkephalin (L-EK), neurotensin (NT), arginine vasopressin (AVP), renin activity (PRA) and angiotensin Ⅱ (AT Ⅱ ) were measured by radioimmunoassay in 60 normal persons and 120 hypertensive patients. There were lower levels of ?-EP and L-EK (P
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We studied the effects of the selective thromboxane synthetase inhibitor, dazoxiben (UK-37 248), on the reperfusion arrhythmias induced by ischemia-reperfusion in rats. The results indicated that the administration of dazoxiben (2.5 mg/kg i.v.) 15 min prior to coronary occlusion significantly decreased the incidence of ventricular fibrillation induced by 5 inin ischemia and reperfusion from 11/13 in control to 4/13 in drug-treated (P
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The present study investigated the effect of BN50739, a new PAF antagonist, on the early reperfusion-induced arrhythmia in anesthetized SD rats, and by this to elucidate the role of PAF in the mechanism of early reperfusion-induced arrhythmia. The results indicated that the BN50739 group had lower incidences of premature ventricular contraction (PVQ, ventricular tachycardia (VT) and ventricular fibrillation (VF) during reperfusion than the control group (58.3% vs 66.7%, 50% vs 77.8%, and 16.7% vs 88.9%). But only VF incidence had a significant difference. Also the BN50739 group had shorter duration of VT+VF and of total arrhythmias than control group (58.8+171.9 vs 296.4+291.4, and 76.4+173.9 vs 361.8 + 286.5, P
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Plasma levels of artial natriuretic polypeptide (ANP), cyclic GMP (cGMP), renin activity (PRA), angiotensin II (AT II) and arginine vasopressin (AVP) were measured by radioimmunoassay in 30 patients with acute heart failure (AHF), 30 chronic heart failure (CHF), 30 chronic atrial fibrillation (CAF) and 27 paraxysmal atrial fibrillation and supraventricular tachycardia (SVT). The results showed that plasma ANP and cGMP levels in all these four groups were significantly higher than those in the normal group (P