ABSTRACT
Objective To investigate the expression of microRNA‐101 (miRNA‐101 ) in human gastric cancer ,and to explore its effects on proliferation ,migration and invasion of gastric cancer cell . Methods The expression of miRNA‐101 in 28 human gastric cancer tissues ,human gastric cell lines BGC‐823 , SGC‐7901 , MKN‐45 , AGS and human normal gastric epithelial cell line GES‐1 were determined by real time polymerase chain reaction (PCR) .Recombinant miRNA‐101 adenovirus vector was constructed . The effects of miRNA‐101 on gastric cancer proliferation was detected with cell proliferation assay .The ability of gastric cancer cell migration and invasion was assessed with Transwell assay .Gastric xenograft cancer model was established in BALB /c nude mice and the tumor size was compared .The t test was used for the statistical analysis .Results The expression of miRNA‐101 in gastric cancer tissues was 0 .661 ± 0 .396 ,which was lower than that of corresponding para carcinoma tissues (1 .128 ± 0 .697) ,and the difference was statistically significant (t = 10 .091 , P < 0 .01) .The expression of miRNA‐101 in normal gastric epithelial cell line GES‐1 was higher than those of gastric cancer cell lines BGC‐823 ,SGC‐7901 , MKN‐45 and AGS . There was significant suppression role of miRNA‐101 on MKN‐45 cells proliferation , and which also had inhibition role on cell migration and invasion of gastric cancer cell lines BGC‐823 ,SGC‐7901 ,MKN‐45 and AGS .At five weeks after MKN‐45 gastric xenograft cancer nude mice model established ,the tumor size of Ad‐miRNA‐101 group ((333 .56 ± 46 .71) mm3 ) was smaller than that of Ad‐enhanced green fluorescent protein (EGFP) group (806 .41 ± 51 .83) mm3 ,and the difference was statistically significant (t = 21 .431 , P < 0 .01 ) .Conclusion In gastric tissues and cells ,miRNA‐101 is a tumor suppressive miRNA and its downregulated expression involved in the genesis and development of gastric cancer ,which may be a new target of biological target therapy in gastric cancer .
ABSTRACT
Objective To compare the diagnostic efficacies of narrow-band imaging (NBI) in distinguishing neoplastic from non-neoplastic colorectal lesions with routine endoscopy and with magnifying endoscopy. Methods Patients with colorectal lesions detected by NBI from September 2008 to February 2010 were enrolled in the study. These lesions were classified by pit pattern and capillary pattern, which was then assessed by reference to histopathology. Results A total of 100 patients with colorectal lesions were enrolled, and the lesions were observed by NBI with ordinary endoscopy (n =64) and NBI with magnifying endoscopy (n =36), respectively, and 7 cases (5 in NBI with ordinary endoscopy and 2 in NBI with magnifying endoscopy) which did not meet the diagnostic criteria were excluded. The overall diagnostic accuracy of NBI endoscopy in distinguishing neoplastic from non-neoplastic colorectal lesions was 91.4% ( 85/93 ), in which NBI with ordinary endoscopy and magnifying endoscopy was 89. 8% (53/59) and 94. 1% (32/34),respectively, with both significantly higher than that of conventional colonoscopy reported in the literature (79. 1% ) (P ＜ 0. 05 ). However, no significant difference was detected between 2 methods ( P ＞ 0. 05 ).Conclusion Similar with NBI magnifying endoscopy, NBI endoscopy without high magnification may also be useful to distinguish neoplastic from non-neoplastic colorectal lesions.