ABSTRACT
Objective To construct a novel M.tb DNA vaccine (p846) co-expressing mycobacterial triple antigens including Rv3615c, Mtb10.4 and Rv2660c, and evaluate its cellular immune response and protective efficacy against tuberculosis infection in BALB/c mice. Methods We constructed the p846 by using the cloning technology. The 6- to -8-week old female BALB/c mice were randomly divided into 4 groups: p846, pcDNA3.1, PBS and the BCG group. All mice were administrated intramuscularly with 50 μg recombinant plasmids at 0, 2, 4, 6 week. A single dose of BCG was injected subcutaneously in the BCG group. Two weeks after the final immunization, 10 mice in each group were used for cell proliferation, ELISPOT and FCM assay, BCG challenge experiment and HE staining of lung were performed at 4, 6 weeks later, respectively. Results The p846 vaccine could effectively induce the specific T cell proliferation(P < 0.001) and increase the numbers of IFN-γ+T cells(P <0.001), compared with those in the PBS group and the vector conreol group. The mouse lung tissue presented very mild lung inflammation in the p846 group, compared with other groups. Conclusion Vaccine p846 could not only induce strong cellular immune response, but also efficiently protect BALB/c mice against M.tb infection.
ABSTRACT
Objective To investigate the effect of sanbai granules on the patients with leucoderma.Methods 180 cases with leucoderma were selected and divided into two groups.90 cases in the control group were treated by Methoxsalen Tablets 10mg,1 times a day,2h sunlight after the medication,appropriate amount external of Compound Kaliziran tincture and Halometasone.90 cases in the experiment group were treated on the base of the control group with Sanbai granules.The activity of tyrosinase,amount of melanin in the serum,clinical effect and amount of skin regeneration between two groups were compared.Results Compared with the control group, the activity of tyrosinase and melanin in serum of the experiment group were higher (P<0.05),the clinical effect rate were higher(P<0.05),number of skin regeneration piece was higher(P<0.05).Conclusion Sanbai granules can effectively improve the activity of tyrosinase and melanin in patients with leucoderma, and it has important significance for the treatment of leucoderma.