ABSTRACT
Objective To investigate the protective effects of ethanol extract of calonyction acculeatum beans( CABE) on rats with acute lung injury ( ALI ) . Methods The ALI model was induced via intravenous injection with 5 mg · kg-1 lipopolysaccharide(LPS). The expression of nuclear factor-кB p65(NF-кB65) was determind by immunohistochemistry(IHC), and the content of interleukin-1β( IL-1β) in the lung tissue was detected by enzyme-linked immunosorbent assay ( ELISA ) . Meanwhile,lung tissue histopathology,the wet-dry weight ratio of lung and the activity of myeloperoxidase(MPO) in the lung tissues were observed. Results Compared with the model group, CABE lessened the lung injury, decreased IL-1β level, reduced the lung dry-wet weight ratio and activity of MPO,and down-regulated the expression of NF-кB/p65(P<0. 05). Conclusion CABE has remarkedly protective effect on ALI induced by LPS in rats. One of the mechanisms may be related to reducing the secretion of IL-1β and interfering with the activity of NF-кB.
ABSTRACT
Objective:To explore the anti-inflammatory effects and the underlying mechanisms of ethanol extract of Ageratum cony-zoides. L. from Guangxi. Methods:The auricle edema model was induced by dimethylbenzene in the mice and the paw edema model was induced by carrageenan respectively in the mice and rats to study the anti-inflammatory effects of ethanol extract of Ageratum cony-zoides. L. from Guangxi. The content of malondiadehyde (MDA) and proateglandin E2 (PGE2), and the activity of superoxide dis-mutase( SOD) in the mouse edema paw was measured. The contents of tumour necrosisfactor-α ( TNF-α) , interleukin-1β ( IL-1β) and interleukin-6(IL-6) in the rat serum were detected as well. Results:Compared with the model control group, the ethanol extracts of Ageratum conyzoides. L. from Guangxi could remarkably inhibit auricle edema in the mice and paw edema in the mice and rats( P<0. 05 or P<0. 01), the inhibition ratio for high, medium and low dosage group(6. 0, 3. 0, 1. 5 g·kg-1)was 29. 24%,16. 42% and 11. 21% in the auricle edema mice and 28. 66%,18. 79% and 13. 13% in the paw edema mice , respectively. It could remarkably re-duce MDA and PGE2 content and enhance the activity of SOD in the mouse inflammatory tissue(P<0. 05 or P<0. 01). In the paw e-dema rats, the inhibition ratio for high, medium and low dosage group(4. 5,2. 3, 1. 2 g·kg-1)was 43. 69%, 36. 01% and 23. 29%at the 3rd h, respectively , and it also could remarkably reduce serum TNF-α, IL-1βand IL-6 content(P<0. 05 or P<0. 01). Con-clusion:The ethanol extracts of Ageratum conyzoides. L. from Guangxi show significantly anti-inflammatory effects, and the mecha-nisms may be related to the ability of scavenging oxygen free radicals and reducing the release of inflammatory cytokines and proinflam-matory cytokines.