ABSTRACT
OBJECTIVE@#Analyse the effect of the treatment of infection in posterior tympani cavity in 168 cases with chronic otitis media.@*METHOD@#Infection was eliminated in posterior tympani cavity of all 168 cases after surgical procedures of otitis media, and 102 tympanoplasty with autoplastic bone were operated in all cases. 22 tympanoplasty were operated without mastoidectomy, 53 with transmastoid approach, 27 with mastoidectomy and tympanoplasty.@*RESULT@#All cases were followed up for more than one year. 160 cases were cured, the effective rate was 95% and no recurrence appeared except for 8 cases. The average threshold of hearing improvement of 87 cases > 15 dB, the effective rate was 85%.@*CONCLUSION@#To eliminate infection, the exploratory surgery of posterior tympani cavity should be done during surgical procedures of otitis media, for improving the effect of the treatment of otitis media.
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Chronic Disease , Ear, Middle , General Surgery , Otitis Media , General Surgery , Tympanoplasty , MethodsABSTRACT
OBJECTIVE@#To establish a rapid method for EBV detection with loop-mediated isothermal amplification (LAMP), and to make it as a clue for early diagnosis of nasopharyngeal carcinoma cancer.@*METHOD@#EBV DNA was fast extracted from samples after boiling, while the whole detection will be finished within an hour with specific amplification of EBV gene by LAMP.@*RESULT@#High specificity was shown from EBV detection of 33 clinical samples. Comparing with PCR, LAMP is more simple and convenient to perform under isothermal conditions, and require no special apparatus, thus, it is more economical and practical.@*CONCLUSION@#LAMP analysis of EBV may be an efficient and easy way for clinical diagnosis of nasopharyngeal carcinoma.
Subject(s)
Humans , DNA Primers , DNA, Viral , Herpesvirus 4, Human , Genetics , Molecular Sequence Data , Nasopharyngeal Neoplasms , Diagnosis , Nucleic Acid Amplification Techniques , Sensitivity and SpecificityABSTRACT
OBJECTIVE To evaluate the roles of p15,p27 gene protein and expression in nasopharyngeal carcinoma(NPC).METHODS The EnVision immunohistochemical method was used to detect the expression of p15,p27 gene protein in nasopharyngeal carcinoma tissues of 43 NPC cases and non-tumor nasopharyngeal tissues of 21 cases.RESULTS ① The positive expression rates of p15,p27 gene protein were 65%,68% in NPC respectively.There were significant differences between NPC and non-tumor group(P0.05).③The positive expression of p15 gene protein was correlated to the positive expression of p27 gene protein(P