ABSTRACT
Objective To analyze the CT features of osteosarcoma and chondrosarcoma and to explore the value of CT differential diagnosis.Methods The CT findings of 35 cases with osteosarcoma and 22 cases with chondrosarcoma confirmed by operation and pathology were analyzed retrospectively.P<0.05 for the difference was statistically significant.Results Periosteal reaction, Codman triangle, radial spicule, type Ⅰ and Ⅳ calcium-like density, long tubular soft tissue mass around greater than 1/2, the ratio of tumor CT to muscle CT (△T/△M) was statistically significant.Of all the above image performance, the sensitivity and specificity of the type I calcium-like density were the highest, while those of the type Ⅳ calcium-like density were the lowest.Conclusion The osteosarcoma is more likely to have the signs of periosteal reaction, Codman triangle, radial spicule and type Ⅰ calcium-like density, while the chondrosarcoma is more likely to have the signs of type Ⅳ calcium-like density.
ABSTRACT
Cellular senescence is one of the important steps against tumor. This study was to observe the characteristics of boningmycin induced senescence of human tumor cells. MIT method and clone formation assay were used to detect the growth-inhibitory effect. Cellular senescence was detected with senescence-associated beta-galactosidase staining. Cell cycle distribution and accumulation of intracellular reactive oxygen species (ROS) were analyzed with flow cytometry. Protein expression was detected by Western blotting. The results showed that the growth-inhibitory effect of boningmycin was obviously stronger on human oral epithelial carcinoma KB cells than that on non-small cell lung cancer A549 cells. Comparison to the similar action of doxorubicin, that boningmycin induced the features of cellular senescence in both cell lines, its due to the arrest at G2/M phase and an increase of ROS level. The molecular senescence marker P21 increased significantly after boningmycin treatment at a dosage of 0.1 micromol x L(-1), whereas a higher concentration of it induced apoptosis. The results indicated that cellular senescence induced by boningmycin was one of its mechanisms in tumor suppression.