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Objective To establish the method for simultaneous determination of six index components in the water extract of Weile Prescription;To optimize the water extraction process.Methods UPLC-MS/MS was used with Waters CORTECS C18 column(2.1 mm×100 mm,1.6 μm)as the chromatographic conditions;the mobile phase was 0.1%formic acid water-acetonitrile with gradient elution;the flow rate was 0.25 mL/min;the column temperature was 40℃;the sample volume was 2 μL.Electrospray negative ion source,positive and negative ion switching multi-reaction monitoring(MRM)mode were detected.Taking the content of six index components(gallic acid,vitexin,paeoniflorin,naringin,hesperidin and glycyrrhizic acid)and extraction rate as evaluation indexes,the weight coefficient of each index was determined by G1-entropy weight method,and the optimum parameters of extraction process were determined by orthogonal experiment design with the amount of water,extraction time and extraction times as investigation factors.Results There was a good linear relationship of the six components in the water extract of Weile Prescription in the concentration range(r>0.999),and the average recovery rate was 96.83%-102.56%,RSD<4.0%.The best technological parameters were as follows:Chinese decoction pieces were soaked in 12 times of water for 2 h,and extracted twice,each time for 1.5 h.Conclusion The UPLC-MS/MS method established in the study for simultaneous determination of six components in Weile Prescription is rapid,simple and sensitive,and the optimized extraction process is stable and feasible,which provides experimental basis for the development and research of the preparation.
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OBJECTIVE:To observe therapeutic efficacy of candesartan combined with Sulodexide soft capsules in the treatment of persistent proteinuria of diabetic nephropathy. METHODS:180 patients with persistent proteinuria of diabetic ne-phropathy were randomly divided into control group and trial group,with 90 cases in each group. Control group was given can-desartan,8 mg/time,once a day;trial group was additionally given Sulodexide soft capsules on the basis of control group,250 LSU/time,twice a day. The indexes levels of both groups were compared before and after treatment,including systolic blood pressure(SBP),diastolic blood pressure(DBP),serum creatinine(Scr),blood urea nitrogen(BUN),urinary albumin excre-tion rate (UAER),24 hour urinary albumin (24hU-Alb) and urinary 2-microglobulin (β2-MG),α1 microglobulin (α1-MG), urine N-acetyl-β-D glucoside enzyme(NAG)and serum cystatin C(Cys-C). RESULTS:After treatment,there was no statisti-cal significance in the levels of SBP,DBP,Scr,BUN and UAER between 2 groups(P>0.05);24hU-Alb level of observation group was significantly betRter than that of control group,with statistical significance (P<0.05);after treatment,β2-MG,α1-MG,NAG and Cys-C of 2 groups were all improved significantly,and the trial group was more significant then the control group,with statistical significance (P<0.05). CONCLUSIONS:Candesartan combined with Sulodexide soft capsules in the treatment of persistent proteinuria of diabetic nephropathy can effectively improve renal function and reduce urinary microalbu-min.
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0. 05). The mean survival time in Iodine-125 stent group was 10. 5 months prominently longer than those in group A 4. 4 months (P