ABSTRACT
Objective: To investigate the effect of remifentanil combined anesthesia on serum cytokines and oxidative stress indices in patients undergoing laparoscopic surgery for colon cancer
Study design: Experimental study
Place and duration of study: Department of anesthesiology, Yuhuangding Hospital affiliated to Qingdao University, Yantai, China, from may 2016 to march 2018
Methodology: A total of 154 patients undergoing laparoscopic surgery for colon cancer were randomly divided into control group and observation group, with 77 cases in each group. Control group received fentanyl combined anesthesia, and observation group received remifentanil combined anesthesia. Levels of serum cytokines IL-8, IL-6, CRP, TNF- alpha; and the levels of oxidative stress indices SOD, MDA, CAT, and GSH on the first day after operation were compared. Occurrence of adverse reactions during anesthesia recovery was observed and recorded in both groups
Results: On the first day after surgery, levels of serum cytokines IL-8, IL-6, CRP, TNF- alpha; and MDA in the observation group were lower than those in the control group [all p<0.001]; levels of serum SOD, GSH, and cat in the observation group were higher than those in the control group [all p<0.001]. The frequency of adverse reactions such as nausea and vomiting, chills, restlessness, cough, and tachycardia in the observation group was lower than that in the control group [p=0.029, 0.016, 0.009, 0.025, and 0.003, respectively]
Conclusion: Compared with fentanyl combined anesthesia, the remifentanil combined anesthesia can significantly reduce serum levels of cytokines IL-8, IL-6, CRP, TNF- alpha; and oxidative stress level, and is, therefore, more secure for patients undergoing laparoscopic surgery for colon cancer
ABSTRACT
Objective To evaluate the effects of ischemic postconditioning (IPO) on c-fos protein expression during renal ischemia-reperfusion (I/R) in rats.Methods Seventy-five adult male Sprague-Dawley rats,aged 8-12 weeks,weighing 200-250 g,were randomly allocated into 3 groups (n =25 each) using a random number table:sham operation group (group S),I/R group and IPO group.Renal I/R injury was induced by clamping the bilateral renal pedicles for 1 h followed by 24 h of reperfusion in I/R and IPO groups.Five animals were sacrificed at 1,3,6,12 and 24 h of reperfusion and the left kidneys were removed for microscopic examination and for determination of the expression of c-fos protein by immunohistochemistry.Results Compared with S group,the expression of c-fos protein was significantly up-regulated at each time point during reperfusion in I/R and IPO groups.Compared with I/R group,the expression of c-fos protein was significantly down-regulated at each time point during reperfusion in group IPO.The pathologic changes were significantly attenuated in group IPO as compared with group I/R.Conclusion The mechanism by which IPO attenuates renal I/R injury is related to down-regulation of c-fos protein expression in the renal tissues of rats.
ABSTRACT
Objective To investigate the effect of acute hyperventilation on the cerebral function of dogs with acute intracranial hypertension.Methods A total of 16 dogs with acute epidural hematoma were randomly divided into four groups.Group A,the group control,were ventilated and PETCO2 was maintained at 35-45mmHg.Groups B,C and D were hyperventilated and PETCO2 was maintained at 28-35,20-27 and below 20mmHg,respectively,for 1h.Jugular vein blood was taken to determine S100B and CRP content at preoperation(T1),2h(T2) post-ventilation,6h(T3),12h(T4),24h(T5) and 48h(T6).Results S100B and CRP contents in experimental group dogs increased,with a significant difference from that before operation(P