ABSTRACT
Background: The follicular growth and development may be affected by abused drugs. Nandrolone decanoate [ND] as an anabolic androgenic steroid can damage the morphological and functional features of the ovary and may lead to reproductive failure
Objective: This study was designed to evaluate the effects of synchronized and non-synchronized administration of Human Menopausal Gonadotropins [hMG] with ND on ovarian tissue and level of sex hormones in the adult female rat
Materials and Methods: Forty adult female Sprague Dawley rats were divided into eight groups. The five experimental groups received 3 and/or 10 mg/kg of ND synchronized and non-synchronized with 10 IU of hMG and hMG alone. The two shams and control groups received solvents of ND and hMG. The animals' serum levels of Follicle-stimulating hormone, Luteinizing hormone, progesterone and estrogen and the weight, volume and dimensions of the ovaries were measured. The ovaries were prepared for apoptosis assessment and morphological study
Results: The ovarian volume and sex hormones in the experimental groups were decreased, but ovarian weight and dimensions didn't change. The rate of apoptosis was increased in the experimental groups as follows; a low and high dose of ND synchronized with hMG 48.80+/-18.70 and 65.20+/-14.20 respectively vs. Sham 1, 33.20+/-17.80, a low and high dose of ND non-synchronized with hMD 55.80+/-17.20 and 75.20+/-14.30 respectively vs. Sham 2, 31.60+/-32.40 groups, p>/=0.01. Follicular and stromal cells were damaged in the experimental groups except for the hMG group
Conclusion: Administration of ND decreased the serum level of Luteinizing hormone, Follicle-stimulating hormone, progesterone and estrogen and damaged ovarian tissue irreversibly and irreparably and hMG cannot prevent the destruction of the follicles in the adult female rats. This can be a serious warning to women who abuse ND
ABSTRACT
Background: Nandrolone decanoate [ND] is an anabolic androgenic steroid [AAS] which influences the ovarian structure and function. We assessed the effects of ND on the ovarian volume, number of primordial follicles, and level of hormones and also evaluated the modulatory effects of gonadotropins on the histopathological changes imposed by the administration of ND
Methods: Six groups of Sprague-Dawley adult female rats [n=30] were used. The experimental rats were injected intraperitoneally with 3 and 10 mg/kg ND with or without human menopausal gonadotropin [hMG], 10 IU weekly for one month. The vehicle and control rats were administered olive oil and saline, respectively, for the same period of time. The ovarian volume and number of primordial follicles were estimated by stereological methods
Results: The results showed a decrease in the ovarian volume, number of primordial follicles, and level of gonadotropins in the ND-treated animals compared with the vehicle groups. In the rats treated with 3 mg/kg of ND with hMG, an increase in the ovarian volume and number of primordial follicles was shown as compared to the rats treated with the same dose of ND without hMG
Conclusion: ND exerted detrimental effects on the dimensions of the ovary, number of follicles, and level of sex hormones. However, hMG, prevented the harmful effects of ND [at least in a low dose] on the ovarian follicles
ABSTRACT
In traditional medicine zingiber officinale used to regulate female menstural cycle and treat male infertility. Recent studies have suggested the possible role of ginger extract in improving the testicular damage of busulfan. The aim of this study was to evaluate the effects of zingiber officinale on the sperm parameters, testosterone level and the volume of the testes and seminiferous tubules by stereological methods. Fifty rats were divided into four groups. All the rats were given a single intraperitoneally injection of 5mg/kg busulfan solution. The first group was kept as busulfan control, while the other groups were orally administrated ginger extract in graded doses of 50, 100 and 150mg/kg b.wt, for 48 consecutive days. At the end, all animals were anesthetized and their testes and vas deference were removed, fixed, embedded, and stained. The volume of testes and seminiferous tubules were estimated by cavalieri methods. The result showed, that zingiber officinale increased the volumes of seminiferous tubule in 100mg/kg treated group compared to control group. Sperm count [706x10[5] and 682x10[5]] and the level of testosterone [50.90 ng/mL and 54.10 ng/mL] enhanced in 100 mg/kg and 150 mg/kg treated groups compared to control group [p=0.00]. It seems that zingiber officinale stimulate male reproductive system in induce busulfan infertility