ABSTRACT
[Absract] Objective To explore a modified method for establishing a rabbit model of B16F10 cells and the factors in tumorigenesis as well as to observe contrastenhanced ultrasonography (CEUS) in qualitative and quantitative evaluation of dynamic circulation pattern of choroidal melanoma.Methods Tumor fragments of choroidal melanoma were implanted into the suprachoroidal space of the right eyes in 10 rabbits,with the left eyes as the controls.After 4 weeks,rabbits eyes were harvested for examined using ultrasonic imaging,color doppler flow imaging,and CEUS,and the images were analyzed with SonoLiver software.Results The proportion of rabbits with choroidal melanoma formation was 80.0％,with 2 cases dead.The maximum major diameter of the tumor was (0.92 ± 0.28) cm and (0.66 ± 0.30) cm.The maximum intensity,rise time,time to peak,mean transit time and area under curve was (263.56 ±143.33)％,(5.01 ± 1.42)s,(6.17 ±2.18)s,(18.01 ±7.56)s,(4505.13 ± 1491.75)％ respectively,while the rising slope and descending slope was 59.48 ± 75.18 and-7.21 ±5.76 accordingly.And the peak intensity,area under curve and descending slope of choroidal tumor was larger than those of normal eyes (all P ＜ 0.05),while no significant difference was found in rise time,time to peak,mean transit time and rising slope (all P ＞ 0.05).Conclusion Suprachoroidal implantation of tumor fragments can facilitate the formation of the stable animal model of choroidal melanoma.And CEUS application has a great clinical value in qualitative and quantitative evaluation of blood circulation in melanoma.