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Mitochondria is an important organelle in mammalian cells with multiple functions,such as ener-gy production and cell homeostasis maintaining. It is known that hundreds of diseases are associated with mi-tochondrial defects. The studies show that the exoge-nous mitochondria can directly enter mammalian cells in vitro, and they also can quickly transform into ani-mal tissues by local or intravenous injection. Current-ly, it has raised a new therapeutic strategy for mito-chondrial diseases, called mitotherapy, which trans-plants exogenous functional mitochondria into mito-chondria-defective cells. The mitochondria in recipient cells play their own roles, including energy produc-tion,maintaining free radical balance,and cell viabili-ty recovery. Since there is no effective method for mito-chondria-related diseases up to now, the mitotherapy will provide a new approach for the prevention and treatment of these diseases.
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Objective To explore the effectiveness and safety of reduced glutathione in the treatment of uremic patients with anemia.Methods From January 2014 to May 2016,84 uremic patients with anemia in our hospital for treatment were selected as the research object and were equally divided into the observation group and control group with 42 patients in each group accorded to the random draw envelope principle.The control group was treated with hemodialysis and oral administration of erythropoietin therapy,the observation group was received the reduced glutathione treatment based on the control group,all patients were observed for 12 weeks.Results There were no serious complications in the two groups.The total effective rate of the observation group and the control group were 97.6％ and 85.7％ respectively.The total effective rate of the observation group was significantly higher than that of the control group (P ＜ 0.05).The serum UA values in observation group and the control group after treatment were significantly lower than that before treatment,the serum Ccr value were significantly higher than before treatment (P ＜ 0.05),At the same time after treatment,the serum UA and Ccr values in the observation group compared with the control group were statistical significance difference (P ＜ 0.05).The RBC and Hb in the observation group after treatment were (3.38 ± 0.34)×1012/L and (94.55 ± 4.92) g/L,and the control group were (2.56 ± 0.38)×1012/L and (73.24 ± 5.39) g/L respectively that were significantly higher than before treatment (P ＜ 0.05),and the observation group were significantly higher than those in control group (P ＜ 0.05).Conclusion Reduced glutathione in the treatment of uremic anemia can improve renal function and anemia status,improve the therapeutic efficacy,its better safety,and has a good value of application and promotion.
ABSTRACT
Objective Postoperative acute pulmonary embolism (PE) is a high risk complication with high mortality.The aim of this study was to analyze the risk factors for postoperative acute PE and the effect of anticoagulation treatment on the complication and prognosis. Methods Ninety four postoperative patients diagnosed with acute PE by spiral CT pulmonary angiography examination from March 2011 to April 2014 were enrolled.The patients were divided into 2 groups.Group A:Postoperative patients without other risk fac-tors of PE (tumor, pregnancy/delivery, heart failure, COPD, long-term bedridden because of internal diseases , hypertension, coronary heart disease, stroke, atrial fibrillation and deep venous thrombosis of lower limb and PE history );Group B:Postoperative patients with other risk factors of PE .The clinical data werecollected and analyzed including the risk factors of PE , examination results when PE oc-curred ( blood pressure, respiratory rate, heart rate, blood gas analysis, the level of serum D-dimer and BNP), method of treatment, bleeding complications and prognose . Results The oxygen pressure of group A was obviously higher than that of group B ( 87.36 ±23.06 mmHg vs 74.43±24.7 mmHg, P<0.05); the level of serum BNP in group A was lower than that in group B ( 945.64 ±1407.64 pg/mL vs2639.143±4070.375 pg/mL, P<0.05).The rate of anticoagulant therapy was significantly lower in group B than that in group A (90%vs 100%P<0.05) .The mortality of group B was higher than that of group A ( P<0.05) .The therapy method of anticoagulant and thromboly-sis had positive correlation with bleeding complications ( P<0.05) .The therapy method of anticoagulant had negative correlation with the mortality of acute PE, while the tumor, increase in serum BNP increasing , hypoxemia and hypotension had positive correlation with the mortality of acute PE (P<0.05). Conclusion The therapy method of anticoagulant and thrombolysis can increase the risk of bleeding complications of postoperative patients diagnosed with acute PE , while the therapy method of anticoagulant can reduce the risk of death of these patients.The tumor, increase in serum BNP , hypoxemia and hypotension can increase the risk of death .
ABSTRACT
Objective Postoperative acute pulmonary embolism (PE) is a high risk complication with high mortality.The aim of this study was to analyze the risk factors for postoperative acute PE and the effect of anticoagulation treatment on the complication and prognosis. Methods Ninety four postoperative patients diagnosed with acute PE by spiral CT pulmonary angiography examination from March 2011 to April 2014 were enrolled.The patients were divided into 2 groups.Group A:Postoperative patients without other risk fac-tors of PE (tumor, pregnancy/delivery, heart failure, COPD, long-term bedridden because of internal diseases , hypertension, coronary heart disease, stroke, atrial fibrillation and deep venous thrombosis of lower limb and PE history );Group B:Postoperative patients with other risk factors of PE .The clinical data werecollected and analyzed including the risk factors of PE , examination results when PE oc-curred ( blood pressure, respiratory rate, heart rate, blood gas analysis, the level of serum D-dimer and BNP), method of treatment, bleeding complications and prognose . Results The oxygen pressure of group A was obviously higher than that of group B ( 87.36 ±23.06 mmHg vs 74.43±24.7 mmHg, P<0.05); the level of serum BNP in group A was lower than that in group B ( 945.64 ±1407.64 pg/mL vs2639.143±4070.375 pg/mL, P<0.05).The rate of anticoagulant therapy was significantly lower in group B than that in group A (90%vs 100%P<0.05) .The mortality of group B was higher than that of group A ( P<0.05) .The therapy method of anticoagulant and thromboly-sis had positive correlation with bleeding complications ( P<0.05) .The therapy method of anticoagulant had negative correlation with the mortality of acute PE, while the tumor, increase in serum BNP increasing , hypoxemia and hypotension had positive correlation with the mortality of acute PE (P<0.05). Conclusion The therapy method of anticoagulant and thrombolysis can increase the risk of bleeding complications of postoperative patients diagnosed with acute PE , while the therapy method of anticoagulant can reduce the risk of death of these patients.The tumor, increase in serum BNP , hypoxemia and hypotension can increase the risk of death .
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Objective To observe the effects of force signals and inflammatory cytokines on the expressions of functional proteins during the differentiation of periodontal ligament cells(PDLCs) into osteoclasts. Methods The caries-free premolars that needed to be removed for orthodontic treatment were collected,human periodontal ligament cells were cultured in vitro.Human PDLCs were exposed to inflammatory cytokines including interleukin(IL)-1β,-6,-23,and tumor necrosis factor alpha(TNF-α). Cyclicmechanical tension with a maximum 5% elongation for different durations(0,2,4,8,12,and 24 hours) were applied. Then the expressions of signaling molecules related to osteoclastogenesis(OPG) and receptor activated nuclear factor κB ligand(RANKL) were determined at protein levels by Western blotting. Results Inflammatory cytokines improved the expressions of osteoclastgenesis regulators in hPDLCs,while cyclic-tension force reduced their expressions. However,the combined effect of inflammatory cytokines and cyclic-tension force resulted in high expressions of osteoclastgenesis regulators. Conclusion Inflammatory cytokines can promote the expressions of the osteoclastgenic factors,which can not be offset by cyclic-tension force.
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<p><b>OBJECTIVE</b>To investigate the expression and effect of Connexin43 (Cx43) on tensile tension-stimulated osteogenic transcription factors of human periodontal ligament fibroblasts (hPDLFs).</p><p><b>METHODS</b>After hPDLFs were treated with 5% elongation tension for 1 h, 2 h, 4 h, 8 h, and 24 h, we examined the expressions of Cx43, Osterix, and RUNX2 at the mRNA level. After Cx43 expression was suppressed by siRNA or 18α-GA, the changes The mRNA in hPDLFs of Osterix and RUNX2 were observed.</p><p><b>RESULTS</b>The expressions of Cx43, Osterix, and RUNX2 mRNA in hPDLFs increased in a time-dependent fashion following tensile strain (all P<0.05), with the highest level at 5% elongation for 24 h. After Cx43 expression was blocked by two different methods, the increasing expressions of Osterix and RUNX2 were inhibited.</p><p><b>CONCLUSIONS</b>5% cyclic tension upregulates Cx43 expression and promotes the expression of Osterix and RUNX2 in a time-dependent manner. Cx43 may be involved in the osteogenic response of hPDLFs to mechanical tension.</p>