ABSTRACT
The tumor targeted fluorescent magnetic IR780-Fe3 O4 nanoparticles were prepared for separation and detection of circulating tumor cells ( CTCs ) . These IR780-Fe3 O4 nanoparticles were characterized by electron microscopy, fluorescence spectrometer, and superconducting quantum interferometer. The targeting effect of IR780-Fe3 O4 nanoparticles was analyzed on the tumor and normal cells by confocal microscope and flow cytometry, and the confocal microscope was used to target the location of IR780-Fe3 O4 nanoparticles in MCF-7 cells. The standard curve was drawn and evaluated accorded to the IR780-Fe3 O4 nanoparticles fluorescence intensity of tumor cells after incubation. The results showed that IR780-Fe3 O4 nanoparticles could target a variety of CTCs. Furthermore, cellular localization experiment proved that IR780-Fe3 O4 nanoparticles could target the mitochondria of tumor cells. With the method of coupling magnetic Fe3 O4 nanoparticles, IR780 could well distinguish the tumor and normal cells, which could be used for separating and detecting the CTCs in simulated blood.
ABSTRACT
The tumor targeted fluorescent magnetic IR780-Fe3 O4 nanoparticles were prepared for separation and detection of circulating tumor cells ( CTCs ) . These IR780-Fe3 O4 nanoparticles were characterized by electron microscopy, fluorescence spectrometer, and superconducting quantum interferometer. The targeting effect of IR780-Fe3 O4 nanoparticles was analyzed on the tumor and normal cells by confocal microscope and flow cytometry, and the confocal microscope was used to target the location of IR780-Fe3 O4 nanoparticles in MCF-7 cells. The standard curve was drawn and evaluated accorded to the IR780-Fe3 O4 nanoparticles fluorescence intensity of tumor cells after incubation. The results showed that IR780-Fe3 O4 nanoparticles could target a variety of CTCs. Furthermore, cellular localization experiment proved that IR780-Fe3 O4 nanoparticles could target the mitochondria of tumor cells. With the method of coupling magnetic Fe3 O4 nanoparticles, IR780 could well distinguish the tumor and normal cells, which could be used for separating and detecting the CTCs in simulated blood.
ABSTRACT
AlM:To determine the group distribution characteristics of lachrymal duct obstruction diseases in major Li Miao minority areas of Hainan province. METHODS: Totally 5 353 residents were selected and researched by randomized cluster sampling in the major Li Miao minority areas of Hainan province. Ocular examination and lachrymal duct flushing were carried out, and questionnaire survey on lachrymal duct obstruction was conducted. The ratio of lachrymal duct obstruction diseases and group distribution characteristics were analyzed based on above research. RESULTS: The prevalence ratios of lachrymal duct obstruction was 4. 47% in major Li Miao minority areas of Hainan province, with 2. 62% in urban area, and 5. 93% in rural area respectively. Prevalence ratios of men and women group were 1. 69% and 6. 39% correspondingly. Difference between the two groups was statistically significant (χ2 = 67. 2821, P = 0. 0000 ). The highest prevalence ratio was 40-69 year-old group, second one was 70-79 year-old group, especially for women in these groups. The prevalence ratios of Ledong, Lingshui, Baisha and Changjiang county were higher than those of Baoting, Qiongzhong county and Wuzhishan city. No significant difference was found between both eyes. CONCLUSlON: ln major Li Miao minority areas of Hainan province, lachrymal duct obstruction mainly occurs in 40-79 year-old patients, with specially higher ratio of women. Statistically, significant difference of the prevalence ratio between urban and rural areas exists. The higher prevalence ratio is attributed to age, gender, geographical location, climate condition, health environment and so on. The prevalence ratio is higher in the dry and windy areas than in the humid and less windy areas.