ABSTRACT
Objective:To establish a method for the determination of erythromycin in erythromycin enteric capsules. Methods:UV spectrophotometry was performed. Sodium hydroxide reacted with erythromycin to obtain a unsaturated ketone having maximum absor-bance at 235 nm. The content of erythromycin in erythromycin enteric capsules was determined by the UV absorbance of the unsaturat-ed ketone. Results: The linear range of erythromycin was 51. 18-307. 08 μg · ml-1 ( r =0. 999 9 ) and the average recovery was 99. 9%(RSD=1. 2%,n=6). Conclusion:The method is simple,accurate,sensitive and reproducible, which can be used for the de-termination of erythromycin in erythromycin enteric capsules.
ABSTRACT
Objective:To establish a HPLC method for the determination of baicalin in Shangsuyu Spray.Methods:A Shim pack CLC ODS column was used. The mobile phase consisted of NH 4H 2PO 4 buffer (3.0) methanol (50∶50). The detective wavelength was 276nm.Results:The linear range was from 1.08 to 20.60?g/ml, r =0.9999. The recovery of the added sample was 100.5%. RSD was 2.5%. This method was easy to operate with accurate result, high sensitivity and good reproducibility.Conclusion:The method can be used for the determination of the contents of baicalin in Shangsuyu Spray.