ABSTRACT
@#ObjectiveTo study the protective effects of tumor necrosis factor α(TNF-α) antibody on liver ischemia reperfusion injury in rats. Methods120 SD rats were randomly divided into three groups, the normal control group(Group A,n=40), ischemia group(Group B,n=40) and TNF-α antibody group(Group C,n=40). After 60 minutes ischemia and followed reperfusion, the animals were killed at 0,1h,3h,6h,12h,serum concentration of alanine aminotransferase(ALT), malondialdehyde(MDA) was meassured and liver histopathology was observed. ResultsAfter ischemia reperfusion, the serum concentration of ALT and MDA in Group B significantly increased (P<0.01),and the hepatic congestion was obvious compared with Group A. Treatment of TNF-α antibody could significantly decrease serum concentration of ALT and MDA in Group C compared with Group B(P<0.01),and relieve hepatic congestion. ConclusionTNF-α antibody can suppress the inflammatory reaction, and protect injury induced by hepatic ischemia reperfusion in rats.
ABSTRACT
@#ObjectiveTo study the effect of aprotinin and tumor necrosis factor alpha(TNF-α) antibody on preventing postoperative abdominal adhesion in mice.Methods96 BALB/c mice were randomly divided into 4 groups:normal saline group(n=24),aprotinin group(n=24),TNF-α antibody group(n=24),and aprotinin combined with TNF-α antibody group(n=24).After an identical peritoneal injury operation,all mice were treated intraperitoneally with normal saline , aprotinin,TNF-α antibody and aprotinin combined with TNF-α respectively at the time of abdominal closure .The mice were killed 20 days after operation,the abdominal incisions and the development of intraabdominal adhesions were observed.Adhesion score was based on an overall assessment of the extent,location,and type of adhesions .ResultsAprotinin combined with TNF-α antibody group showed significantly lower adhesion score and 2-3 grade adhesion(advanced significant adhesion) rate was lower than that of other groups(P<0.01).Conclusions The intraperitoneal administration of aprotinin and TNF-α antibody can inhibit postoperative adhesion formation.