ABSTRACT
Objective:To explore the apoptotic mechanisms of acute myeloid leukemia(AML) cells by a lentivirus mediated-SARI(suppressor of AP-1,regulated by IFN) overexpression system.Methods: The lentiviral vectors of overexpression SARI were transfected into AML cell HL-60 and NB4.Real-time quantitative PCR and Western blot were employed in the following evaluation tests.Both the cells were all divided into blank control group(CON group),empty vector control group(NC group)and SARI overexpression group(SARI group).Western blot was used to detect the expressions of apoptosis-related proteins Bcl-2,Bcl-xl,Bax and apoptotic pathway related proteins CytoC,Caspase8,Caspase9,Caspase3,Actived-Caspase3,PARP.Results:The SARI groups exhibited a high level of SARI mRNA and SARI protein when compared with CON group and NC group(P<0.05).In both the SARI groups,the expression of anti-apoptotic proteins Bcl-2,Bcl-xl were down-regulated and the pro-apoptotic protein Bax was up-regulated.CytoC increased,Caspase8,Caspase9 and Caspase3 decreased,Actived-Caspase3 up-regulated.PARP down-regulated,and PARP spliced variant up-regulated.Conclusion: SARI might promote the apoptosis of AML cells through activing the exogenous and endogenous apoptotic pathways which crossed to exogenous.