Advances of the traditional Chinese medicines modulating endoplasmic reticulum stress / 中国药科大学学报

@#The endoplasmic reticulum is an important organelle for eukaryotic cell protein synthesis, folding and secretion. Perturbation of endoplasmic reticulum homeostasis causes endoplasmic reticulum stress. It has been considered as one of important ways and new strategies to regulate endoplasmic reticulum stress in the treatment of multiple diseases. This paper mainly reviews the advances in the research on traditional Chinese medicines for modulating endoplasmic reticulum stress related to cancer, cardiovascular diseases, neurological diseases, diabetes and other complex diseases in recent years, aiming to provide some clues and references for exploring the characteristics and possible mechanism of traditional Chinese medicines treating related diseases.

Preliminary study on inhibitory effects of sub-MIC matrine alone or in combination with erythromycin On Staphylococcus epidermidis biofilm / 中华微生物学和免疫学杂志

Objective To observe the inhibitory effects of sub-MIC matrine alone and in combination with erythromycin on Staphylococcus epidermidis biofilms and their influences on morphological changes of the biofilms.Methods Minimum inhibitory concentrations (MIC) of matrine and erythromycin against Staphylococcus epidermidis were determined by the serial dilution method,antibacterial activity of matrine combined with erythromycin against planktonic S.epidermidis was evaluated by the checkerboard method.S.epidermidis biofilms were constructed in vitro,XTT reduction assay was used to evaluate influences of sub-MIC matrine alone and in combination with erythromycin on metabolism and adhesion of S.epidermidis biofilms,and scanning electronic microscope(SEM) was applied to observe the morphological and the structural changes of the biofilms.Results The MIC of erythromycin to S.epidermidis was 7.8125 μg/ml,while the MIC of matrine was greater than 1000 μg/ml,besides,a synergistic effect between erythronmycin and matrine on planktonic S.epidermidis was shown (FIC＜0.5).The sub-MIC matrine had no significant inhibitory effect on adhesion of S.epidermidis,and also the combination of the two agents was better than was used alone.However,the sub-MIC matrine had inhibitory effects on metabolism and morphology of S.epidermidis biofilms,and the combination of the two agents was weaker than was used alone.Conclusion Both the sub-MIC matrine and erythromycin had a significant inhibitory effect on S.epidermidis biofilm formation.Combination of the two agents showed synergistic effects on plankton and adhesion of S.epidermidis,but showed no synergistic effect on metabolism and morphology of the biofilms.