ABSTRACT
The most frequently used spermicide Nonoxynol-9 [N-9] in the clinic alters the vaginal flora, which will result in an increased risk of opportunistic infection. So development of a novel spermicidal and microbicidal drug appears to be inevitable. Vaginal local immune is an important part of vaginal flora. Secretory leukocyte protease inhibitor [SLPI], surfactant proteins D [SP-D], and lactoferrin [LF] are anti-microbial molecules with important roles in immune system of female vaginas. To observe effect of a vaginal spermicide nonoxynol-9 [N-9] berberine plural gel on the expression of SLPI SP-D and LF in mice's vaginas. Female BABL/C mice were randomly divided into following 5 groups: normal control group, blank gel group, berberine gel group, 12% N-9 gel group and N-9 berberine plural gel group. Estradiol benzoate at physiological dose was done by hypodermic injection to every group's mice. After 72h, drug gels were separately injected into the mice's vaginas, while immunohistochemistry and Western blot were taken to detect the expression of the 3 indexes in mice's vaginas respectively after 24h and 72h of gel injection. The differences in the three indexes between normal control group and blank gel group were not significant statistically [p>0.05]. The expression of the three indexes in 12% N-9 gel group was decreased compared to that in blank gel group [p<0.05]. The differences in the three indexes between N-9 berberine plural gel group and blank gel group were not significant statistically [p>0.05]. Also, the three index's level of 24h and 72h in sub observation groups after treatment were without statistical significance [p>0.05]. Application of N-9 berberine plural gel had little impact on antimicrobial peptides in normal mice's vaginas
Subject(s)
Female , Animals, Laboratory , Nonoxynol , Berberine , Gels , Mice, Inbred BALB C , Secretory Leukocyte Peptidase Inhibitor , Pulmonary Surfactant-Associated Protein D , Lactoferrin , Anti-Infective AgentsABSTRACT
To observe the differences in psychological status between Bell's palsy (BP) patients and healthy subjects,and to examine the relationship between psychological factors and the severity of BP,we conducted a case-control,multi-center clinical investigation.A total of 695 subjects were assigned to the case group (n=355) and the control group (n=340).House-Brackmann grading system and Facial Disability Index (FDI) were adopted to assess the BP patients; Kessler Psychological Distress Scale (K10) and 16 Personality Factor (16PF) scale were employed to evaluate the psychological distress and personality profiles of all subjects.Two independent samples t test was used to compare the differences between cases and controls,and to compare the differences among different BP patients.Pearson correlation analysis was used to examine the relationship between psychological factors and severity of facial paralysis.The results showed that psychological distress (K1 0) in case group (27.09±5.80) was significantly higher than that in control group (13.43±3.02) (t-37.219,P0.000).The scores of personality factor Warmth (A),Openness to Change (Q1),Self-Reliance (Q2) were lower in cases than in the controls (P＜0.01,P＜0.05,P＜0.05,respectively),whereas the scores of Sensitivity (I),Vigilance (L),Apprehension (O),and Tension (Q4) were significantly higher in cases than in the controls (P＜0.05,P＜0.01,P＜0.01,P＜0.01,respectively).In addition,the psychological distress was significantly higher in female patients,severe (HB score Ⅳ-Ⅵ) patients,and subacute (onset time 72-168 h) patients compared with that in male patients,mild (HB score Ⅰ -Ⅲ) patients,and acute (onset time≤72 h) patients (P＜0.05).The scores of personality factor in female patients,severe patients,and subacute patients were also significantly different from male patients,mild patients,and acute patients (P＜0.05).The result of Pearson correlation analysis showed that psychological factors (K10,personality A,F,L,N,O,Q4) were closely related to HB scores.We are led to conclude that the psychological status between BP patients and healthy people are different; psychological distress and personality factors are closely associated with severity of facial paralysis.
ABSTRACT
In order to observe the effect of Bushenantai recipe on the expression of endometrial leukemia-inhibitory factor (LIF) in mice with embryonic implantation dysfunction (EID), 120 Kun- ruing mice post coition were randomized into three groups: normal control group, model group and traditional Chinese medicine group (TCM group) (n=40 in each group). Uterus was collected on the pregnancy day (Pd) 4, 5, 6 after an intravenous injection of Evan's blue. The endometrium was dyed by Evan's blue and the mean points of response were observed on Pd 5. The expression of LIF mRNA and protein was detected by RT-PCR and immunohistochemistry respectively and analyzed statistically by image system. The results showed that the number of implantation sites in model group was remarkably less than in normal control group and TCM group. There was no significant difference between normal control group and TCM group. The expression of L1F mRNA and protein in model group was delayed. Bushenantai recipe could increase the expression of LIF mRNA and protein in endometria of mice with EID. It was suggested that Bushenantai recipe could improve em- bryo implantation of mice with EID by promoting the endometrial LIF expression and endometrial decidualization.