ABSTRACT
Objective To study the effect of paeonol onexpression of HMGB1and IL-1βand apoptosis of cardiomyocytes in rats after myocardial I/R injury.Methods Fifty-two adult SPF male SD rats were randomly divided into sham operation group,low-dose paeonol group,high dose paeonol group,and I/R injury group(13in each group).The rats in low-dose paeonol group and high dose paeonol group received intraperitoneal paeonol injection before operation(15.30mg/kg,once a day).Serum samples were taken at 2hafter reperfusion.Serum IL-1βlevel was measured by ELISA,myocardial infarction size was measured with TTC staining,expressions of HMGB1,Bcl-2and Bax proteins in myocardial tissue were detected with immunohistochemical staining.Results The myocardial infarction size was significantly smaller in low-dose paeonol group and high dose paeonol group than in I/R injury group(31.04％±2.93％,27.97％±2.84 vs37.23％±3.62％,P＜0.01).The expression levels of IL-1βand HMGB1were significantly higher in I/R injury group,low-dose paeonol group and high-dose paeonol group than in sham operation group and were significantly lower in low-dose paeonol group and high-dose paeonol group than in I/R injury group(P＜0.01).The expression level of IL-1βwas significantly higher while that of Bax protein was significantly lower in low-dose paeonol group and high-dose paeonol group than in in I/R injury group(P＜0.05).Conclusion Paeonol preconditioning can reduce apoptosis of cardiomyocytes,and can thus prevent myocardial I/R injury.
ABSTRACT
Objective To develop a control program running on the magnetic induction escape decompression control timer. Methods The software was developed by VHDL in a modular style under the environment of Quartus II. Results The control software stores decompression profile and accurately controls the commitment of the decompression process. Conclusion The program can work accurately according to the profile selected and meet our needs perfectly.