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Rev. peru. biol. (Impr.) ; 20(2)ago. 2013.
Article in English | LILACS-Express | LILACS, LIPECS | ID: biblio-1522325

ABSTRACT

Bartonellosis and rickettsiosis are commonly reported in Peru. In order to detect Bartonella sp. and Rickettsia sp. in fleas, ticks and lice, specimens from five distinct locations in Peru (Marizagua, Cajaruro, Jamalca, Lonya Grande and El Milagro) were collected and screened for the presence of these bacteria using PCR and later confirmation by DNA sequencing. The specimens collected were distributed in 102 pools (76 Ctenocephalides felis, 2 Ctenocephalides canis, 16Pulex irritans, 5 Pediculus humanus, 2 Rhiphicephalus sanguineus, and 1 Boophilus spp.), whereBartonella was detected in 17 pools (6 of C. felis, 9 of P. irritans, 1 of C. canis, and 1 P. humanus). Also, Rickettsia was detected in 76 pools (62 C. felis, 10 P. irritans, 2 P. humanus, and 2 C. canis). Bartonella clarridgeiae was detected in C. felis, C. canis and P. irritans pools at 5.3%, 50% and 12.5%, respectively. Bartonella rochalimae was detected in one C. felis and two P. irritans pools at 1.3% and 12.5%, respectively. Furthermore, B. henselae was detected in one C. felis pool and one P. humanus pool corresponding to 1.3% and 20%, respectively; andBartonella spp. was also found in 5 pools of P. irritans at 31.3%. Additionally, R. felis was detected in C. felis, C. canis and P. irritans pools at 76.3%, 100% and 37.5%, respectively; and Rickettsia spp. was detected in C. felis, P. irritans and P. humanus pools at 5.3%, 25% and 40%, respectively. These results demonstrate the circulation of these bacteria in Peru


La Bartonellosis y la Rickettsiosis son enfermedades comúnmente reportadas en Perú. Con el propósito de detectar Bartonella sp. y Rickettsia sp. especímenes de pulgas, garrapatas y piojos de cinco localidades del Perú (Marizagua, Cajaruro, Jamalca, Lonya Grande and El Milagro) fueron colectadas y analizadas. Para la detección se usó PCR y una posterior confirmación con secuenciamiento de DNA. Los especímenes colectados fueron agrupados en 102 pools (76 Ctenocephalides felis, dos Ctenocephalides canis, 16Pulex irritans, cinco Pediculus humanus, dos Rhiphicephalus sanguineus, y un Boophilus spp.). Bartonella fue detectada en 17 pools (seis de C. felis, nueve de P. irritans, uno de C. canis, y uno de P. humanus). Rickettsia fue detectada en 76 pools (62 de C. felis, 10 de P. irritans, dos de P. humanus, y dos de C. canis). Bartonella clarridgeiae fue detectada en C. felis (5.3% especímenes), C. canis (50%) y P. irritans (12.5%). Bartonella rochalimae fue detectada en C. felis (1.3%) y P. irritans (12.5%). Además, se detectó B. henselae en C. felis (1.3%) y P. humanus (20%). Bartonella spp. también se encontró en P. irritans (31,3%). Además, se detectó R. felis en C. felis (76.3%), C. canis (100%) y P. irritans (37.5%), y Rickettsia spp. se detectó en C. felis (5,3%), P. irritans (25%) y P. humanus (40%). Estos resultados demuestran la circulación de estas bacterias en el Perú

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