ABSTRACT
DNA barcoding, widely used and applied in identifying animal species, has become one of the hot topics in the study of biology over the past decade; However, it is encountered bottlenecks because none of the available loci work across all plant species. The most candidate DNA barcodes include single-locus barcodes (matK, rbcL, etc.) and multi-locus barcodes (rbcL + trnH-psbA, etc.). Here, we review the development of candidate barcodes and the application in the identification of medicinal plants. We also discuss the feasibility of using the complete chloroplast genome as "super-barcode" and expect to provide a new approach for fast and accurate identifications of traditional Chinese medicinal plants.
ABSTRACT
Aim To observe the timed analgesic effects by butabital (B), acetaminophen(A) and caffeine (C) in combination (BAC), in which the proportion was fixed as1. 25∶ 8. 1∶1. Method Three types of experimental methods, including the tailflicking method, the hot plate method and the pressurizing tail method, weretaken to determine indices at different times after the animals were adminstered(ig) high, median and low BAC dose. Results and conclusion BAC had a stronganalgesic effect in three types of experiments. The effect began 30 min after ad-ministration, arrived maximum at 1 h, decreased at 2 h and disappeared at 4 h.There was a dose-effect relationship between large and little BAC dose.