ABSTRACT
Objective To investigate the chemical constituents of the root of Wrightia pubescens.Methods Compounds were isolated by the combined use of colum chromatography,preparative HPLC and recrystallization,and their structures were identified by their physicochemical and spectroscopic data.Cytotoxic activities of the compounds were evaluated using MTT method in vitro.Re?sults Twelve compounds 1-12 were isolated from the ethyl acetate soluble part from 75% ethanol extract of the root of W.pubescens, and identified as scopoletin(1),coumarin(2),cleomiscosin B(3),mollugin(4),4-hydroxybenzoic acid(5),vanillic acid(6), vanillin(7),4-hydroxymethyl-5-hydroxy-2H-pyran-2-one(8),β-sitosterol(9),β-daucosterol(10),ursonic acid(11),and me?dioresinol(12). Compound 4 showed cytotoxic activity against HepG2 cells in vitro with IC50of 8.0 mg/L. The IC50of compound 11 against MCF-7 and HepG2 cells was 14.7 and 18.2 mg/L,respectively.Conclusion Compounds 1-5,8 and 12 were isolated from the genus Wrightia for the first time. Compounds 6 and 10 were isolated from the title plant for the first time. Compounds 4 and 11 showed cytotoxic activities against tumor cells in vitro.
ABSTRACT
Objective To study the chemical constituents of the male anthotaxy of Populus tomentosa Carr.Methods The compounds were isolated and purified by ODS,silica gel,Sephadex LH-20 column chromatography and recrystallization.Their struc?tures were identified based on the physiochemical properties and spectroscopic data. The antioxidant capacities of some compounds were assayed by a rapid ABTS method.The antitumor activity was tested by the MTT assay.Results Fourteen compounds were isolat?ed from the ethyl acetate part of 70% ethanol extract of the male anthotaxy of P.tomentosa Carr.,and they were identified as pinocem?brin(1),dillenetin(2),chrysoeriol(3),naringenin(4),isosakuranetin(5),apigenin(6),kaempferol(7),apigenin-7-O-β-D(-6″-p-coumaroyl)-glucoside(8),tremuloidine(9),catechol(10),3,3′,4,4′-tetrahydroxybiphenyl(11),coumaric acid(12),ursolic acid (13),and betulonic acid(14),respectively. The results of the ABTS assay showed that 2,6 and 7 could scavenge ABTS+free radicals with the total antioxidant capacity of 0.61,0.14,0.46 respectively.The screening results of antitumor activity in vitro showed that the half inhibitory concentration(IC50)of compounds 1,4 and 5 for adenocarcinomic human alveolar basal epithelial cells A549 was 26.04 mg/L,43.45 mg/L and 24.01 mg/L,for human hepatocellular liver carcinoma cells HepG2 were 13.50 μg/ml,23.80 μg/ml and 9.13 μg/ml,and for human colon carcinoma cells HCT116 was 36.11 mg/L,48.95 mg/L and 32.25 mg/L.Conclusion Compounds 2,3,11,13 and 14 were isolated from the Populus genus for the first time,2-5,10,11 and 13-15 were isolated from the plant for the first time.Compounds 2,6 and 7 displayed the antioxidant activity to a certain extent.Compouds 1,4 and 5 showed varying de?grees of inhibitory effects on tumor cells in vitro.