ABSTRACT
<p><b>OBJECTIVE</b>To investigate the status of bacterial contamination in the shellfish products in Wenzhou.</p><p><b>METHODS</b>One hundred samples were collected and their bacterial populations including the total plate count were investigated.</p><p><b>RESULTS</b>Of the 100 samples collected, 67 samples failed to not meet the national regulations due to bacterial contamination, accounting for 67% of the total samples. Among the contaminated samples, the most serious contamination was caused by coliforms (61.4% of the total plate count with contamination), followed by Salmonella (18.6%), Vibio parahaemolyticus (15.7%), Listeria spp. (4.3%) and others (6%).</p><p><b>CONCLUSION</b>Microbial pollution has become a threat to the marine shellfish products in Wenzhou.</p>
Subject(s)
Animals , China , Colony Count, Microbial , Food Contamination , Food Microbiology , Listeria , Salmonella , Shellfish , MicrobiologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of bacteria in the etiology of chronic prostatitis.</p><p><b>METHODS</b>A total of 162 complete prostate specimens were obtained at autopsy from organ donors (aged 20 -38 yr) who died of non-prostatic diseases. Each of the samples from the peripheral zone of the prostate was divided into two parts, one for routine pathological examination and immunohistochemical studies of interleukin (IL)-1beta, tumor necrosis factor-alpha (TNF-alpha) and the nerve growth factor (NGF), and the other for PCR assay to detect the bacterial 16S rRNA gene (16S rDNA).</p><p><b>RESULTS</b>Fifty-one (31.5%) of the total specimens presented pathological changes of chronic prostatitis, of which 44 had mild focal stromal, 5 mild focal stromal and periglandular and 2 mild focal periglandular inflammation. The positive rate of 16S rDNA was 19.1% (31/162), 51.0% (26/51) in the chronic prostatitis and 4.5% (5/111) in the non-prostatitis specimens (chi2 = 29.783, P < 0.01). In the specimens with chronic prostatitis, the expressions of IL-1beta, TNF-alpha and NGF were significantly higher in the 16S rDNA positive than in the 16S rDNA negative group (P < 0.01).</p><p><b>CONCLUSION</b>Bacterial inflammation may play an important role in the etiology of chronic prostatitis.</p>
Subject(s)
Adult , Humans , Male , Young Adult , Chronic Disease , Genes, rRNA , Interleukin-1beta , Metabolism , Nerve Growth Factor , Metabolism , Prostate , Metabolism , Microbiology , Pathology , Prostatitis , Metabolism , Microbiology , Pathology , RNA, Bacterial , Genetics , RNA, Ribosomal , RNA, Ribosomal, 16S , Genetics , Tumor Necrosis Factor-alpha , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To analyze the degrees on the epidemic foci of Angiostronglus cantonensis and to explore the measurement methods.</p><p><b>METHODS</b>Snails (Pila gigas) were collected from the spots of Wenzhou, Cangnan, Yongjia, Yueqing in Zhejiang province and Minhou, Changle, Ningde in Fujian province. The snails were examined microscopically in order to calculate their infection rates and the average worm number in the positive snails, then taking the product of multiplication of both values as infestation index.</p><p><b>RESULTS</b>The infection rates of the epidemic foci were 10.59% (9/85), 60.74% (181/298), 34.96% (79/226), 32.90% (76/231), 57.50% (184/320), 40.00% (82/205), 17.65% (12/68) and the rates of infectivity were 6.57, 183.54, 121.73, 93.45, 276.36, 76.08, 12.65, respectively.</p><p><b>CONCLUSION</b>The epidemic foci were divided into five ranks (super, high, mid-range, low and non-epidemic foci) according to the value of infestation index which ranked from > 75, 30-75, 5-29, < 5 to 0.</p>