ABSTRACT
OBJECTIVE@#To observe the effect of co-culture cytokine-induced killer cells (CIK) and homologous dendritic cells (DC) on the proliferative activity and phenotype change of the DC-CIK cell and the cell killing activity of leukemia HL-60.@*METHODS@#50 mL cord blood sample was obtained from infants delivered by full term healthy woman and the cord blood mononuclear cells were isolated by density gradient centrifugation. Non-adherent cells were collectedfor the induction culture of CIK, adherent cells were differentiated into mature DC; cultured mature DC was mixed with and CIK in the proportion of 1:5 for 12 d. Killing activity of DC-CIK co-cultured cell on leukemia HL-60 was detected by MTT assay.@*RESULTS@#Compared with CIKs, the co-cultured DC-CIKs presented a markedly higher proliferation and killing activity.@*CONCLUSIONS@#Co-culture of DC-CIK cells led to a significant increase of the proliferation and cytotoxicity of CIK.
Subject(s)
Female , Humans , Apoptosis , Physiology , Cell Differentiation , Physiology , Cell Proliferation , Physiology , Coculture Techniques , Cytokine-Induced Killer Cells , Cell Biology , Cytokines , Metabolism , Dendritic Cells , Cell Biology , Fetal Blood , Cell Biology , HL-60 Cells , Leukemia , PhenotypeABSTRACT
<p><b>OBJECTIVE</b>To assess the therapeutic effect and mechanism of electroacupuncture combined with auricular point tapping and pressing on the obese women with polycystic ovary syndrome.</p><p><b>METHODS</b>Thirty-nine cases of obese women with polycystic ovary syndrome were treated with electroacupuncture combined with auricular point tapping and pressing, body points as Tianshu (ST 25), Fenglong (ST 40), Guanyuan (CV 4) and Siman (KI 14) etc. were selected, and ear points as Kou (mouth), Wei (stomach) and Pi (spleen) etc. were selected. After 3 courses, the therapeutic effect, the body mass index (BMI), the waist circumference (WC) and the changes of the serum insulin (Ins) and testosterone (T) were compared before and after treatment.</p><p><b>RESULTS</b>Of the 39 cases, 10 cases were cured, 25 cases were effective, 4 cases were ineffective, with a total effective rate of 89.7%; there were significant differences in BMI, WC, Ins and T of the patients compared with that before treatment (all P < 0.01).</p><p><b>CONCLUSION</b>Electroacupuncture combined with auricular point tapping and pressing has a good clinical effect on obese women with polycystic ovary syndrome, the treatment mechanism may realized by regulating the serum insulin and the testosterone of the patients.</p>