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Thrombosis is a common complication of heart failure, which seriously affects the life quality of patients.At present, anticoagulant therapy in children with heart failure is still not standardized, and the use of anticoagulants depends more on the experience from adults.However, the etiology and coagulation system development of children are different from those of adults.So the anticoagulant treatment strategy for children with heart failure may be different.This review introduced the changes of coagulation function and anticoagulation therapy in children with heart failure, in order to provide reference for the management of coagulation system and anticoagulation therapy in children with chronic heart failure.
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Congenital heart disease(CHD)is the most common birth defect and is also the leading cause of death in infants and young children. Studying the pathogenesis of CHD and preventing the development of CHD are ma-jor scientific problems that need to be solved urgently. Now,it is known that the occurrence of CHD is caused by multi-ple factors such as genetic factors and environmental factors. In this paper,the genetic mechanisms in the progress of CHD were summarized.
ABSTRACT
Congenital heart disease (CHD) is the most common birth defect and is also the leading cause of death in infants and young children.Studying the pathogenesis of CHD and preventing the development of CHD are major scientific problems that need to be solved urgently.Now, it is known that the occurrence of CHD is caused by multiple factors such as genetic factors and environmental factors.In this paper, the genetic mechanisms in the progress of CHD were summarized.
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[ ABSTRACT] AIM: To investigate the regulatory mechanism of histone acetylation on cardiac hypertrophy in -duced by phenylephrine , and to provide a new idea for preventing and curing hypertrophic cardiomyopathy .METHODS:Phenylephrine was given by continuous subcutaneous injection for modeling cardiac hypertrophy in C 57BL/6 mice.The le-vel of histone H3 lysine 27 acetylation (H3K27ac) in the promoter of cardiac nuclear transcription factor GATA4 and the mRNA expression of GATA4 were identified by chromatin immunoprecipitation (ChIP) and real-time PCR, respectively. Meanwhile, the protein expression of histone H3K27ac, atrial natriuretic peptide (ANP) and α-myosin heavy chain (α-MHC) was determined by Western blot .Cardiac hypertrophy in the mice was observed by HE staining and echocardio-graphy.RESULTS:The results of Western blot showed that the level of histone H 3K27ac in phenylephrine group was sig-nificantly increased compared with normal saline group (P<0.05), and ChIP-qPCR data showed that the level of histone H3K27ac in the promoter of GATA4 was increased significantly in the same samples (P<0.05).The expression levels of GATA4 mRNA and ANP protein in phenylephrine group were apparently increased compared with normal saline group ( P<0.05).However, histone acetylase inhibitor anacardic acid attenuated histone H 3K27 hyperacetylation induced by pheny-lephrine, and downregulated the over-expression of GATA4 and ANP in the heart of the mice (P<0.05).HE staining and echocardiography data showed that phenylephrine apparently increased left ventricular posterior wall thickness and de -creased left ventricular end-systolic diameter and left ventricular end-diastolic diameter , while anacardic acid also reversed these indexes that mentioned above and attenuated cardiac hypertrophy induced by phenylephrine in the mice .CONCLU-SION:Histone acetylation modification imbalance is involved in cardiac hypertrophy induced by phenylephrine , and the histone acetylase inhibitor anacardic acid decreases histone hyperacetylation induced by phenylephrine and attenuates car -diac hypertrophy in the mice .
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It is recognized that the increasing prevalence of diastolic heart failure in adults has signifi-cantly worsen the quality of life and shorten lifespan.However,the assessment and diagnostic criterion of dias-tolic heart failure in children is controversial.The diagnosis and treatment of DHF are mainly based on small samples of clinical research and clinical experience.There is no effective treatment plan of diastolic heart failure from evidence-based medicine.In this article,we review the progress of diastolic heart failure in children in as-pects of its pathological mechanism,clinical manifestations,function evaluation,diagnosis and treatment.
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Objective To investigate that ox-LDL inhibits endothelial differentiation of bone marrow mesenchymal stem cells (MSCs) in rats and its underlying mechanism .Methods Cultured MSCs were divided into four groups:blank groups , ox-LDL groups ( 5 μg/mL ox-LDL ) , ox-LDL +NAC groups ( 5 μg/mL ox-LDL and pre-treated NAC), and negative LDL groups (5 μg/mL nLDL).Cell morphology, endothelial marker and differentiation effi-ciency as well as signal of oxidative and pathway protein were detected after induction by Western blot , real-time PCR.Results MSCs can differentiate into endothelial cells with the expression of endothelial marker vWF , Flk-1 and CD31 at the mRNA and protein level , vascular morphology , ox-LDL obvious inhibited endothelial differentia-tion of MSCs ( P<0.05 ) , but NAC can reverse the inhibition , the amount of ROS in ox-LDL groups was higher than that in ox-LDL+NAC groups ( P <0.05 ) , The expression of phosphorylated Akt decreased distinctly after treatment with ox-LDL( P<0.05 ) , NAC can stimulated its expression close to normal .Conclusions ox-LDL can inhibit endothelial differentiation of MSCs via ROS , NAC in this procese shows inhibition to effect of ox-LDL and Akt signaling also played a critical role .
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There is a trackphenomenon in the development of hypertension and pediatric hypertension has an important predictive value for adult hypertension. However pediatric hypertension is not typical and normal blood pressure is associated with age,sex and height. The blood pressure level,clinical presentation and complication rates of secondary pediatric hypertension are higher than primary pediatric hypertension. So secondary pediatric hypertension could get timely treatment compared to primary pediatric hypertension. This paper focus on the clinical presentation and diagnosis criterion of pediatric hypertension and provide a reference for the pediatrician.
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Objective To detect the effect of ox‐LDL on self‐renewal and Oct‐4 express of MSCs in vitro .Methods MSCs cul‐tured in vitro were divided into 4 groups :blank control(no reagents in culture system ) ,ox‐LDL (1 ,5 ,10 ,20 μg/mL ox‐LDL were added into culture system) ,ox‐LDL+NAC(corresponding ox‐LDL were added into culture system after NAC treatment ) ,negative control(corresponding nLDL were added into culture system ) .Growth curve were drawn at different time ,Oct‐4 ,a stem cell special marker ,were detected by real‐time PCR ,the production of ROS (reactive oxygen species ,ROS) in culture system were measured with electron paramagnetic resonance spectroscopy .Results proliferation of MSCs was inhibited by ox‐LDL ,when concentration of ox‐LDL was more than 5μg/mL ;apoptosis of MSCs appeared as well as attenuated expression of Bcl‐2 ,ox‐LDL generated a signifi‐cant amount of ROS in the culture system ,which was completely prevented by NAC .Conclusion The proliferation and Oct‐4 ex‐pression of MSCs were inhibited by ox‐LDL ,which may be related to increase of ROS in culture system .
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Objective To observe the clinical efficacy of docetaxel and nedaplatin (NDP)in treatment of patients with epithelial ovarian cancer. Methods 50 cases with epithelial ovarian cancer collected in Shifang People’s Hospital from 2009 January to 2010 January were randomly divided into treatment group and control group,each had 25 cases.Treatment group were given docetaxel and nedaplatin,while control group were given carboplatin. The efficacy and adverse reactions in two groups were observed and compared.Results The recent efficacy in two groups were 76.00% and 52.00%, respectively,and the difference was significant (P <0.05 ).Compared with control group, the incidence of leucopenia was 36.00% and thrombocytopenia was 24.00% in treatment group,there was no significant difference between them.The incidences of nausea,vomiting and alopecia in treatment group were 12.00%,8.00% and 44.00%,which lower than that in control group (40%,28% and 64%),the differences between two groups were all significant(P<0.05 ).The two years survival rates in treatment group was 37.5%,and 28% in control group,there was no significant difference between two groups.Conclusion Docetaxel and nedaplatin has good clinical efficacy in treatment of epithelial ovarian cancer.
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Objective To construct the p300 specific siRNA vector in mice and observe its effect on cardiac transcription factor,GATA4,for further study of the effect of p300-mediated histone acetylation on heart growth. Methods Three recombinant plasmid vectors ( p300 RNAi1,p300 RNAi2,and p300 RNAi3s) ,designed and constructed according to the conservative sequence of mice p300,were respectively tansfected into in vitro cultured cardiac muscle cells of suckling mice. Expression of p300 and GATA4 at mRNA and protein levels was detected by RT-PCR and immunofluorescence,respectively. Results The expression level of p300 mRNA was not significantly decreased in pSOS-HUS and p300 RNAi2 groups,but significantly decreased in p300 RNAi1 and p300 RNAi3 groups ( 0. 220 8 ? 0. 020 0 and 0. 170 8 ? 0. 040 0 vs 0. 509 5 ? 0. 030 0,P