ABSTRACT
Objective To establish a HPLC method for the determination of ferulic acid in Xuefu Zhuyu Decoction. Methods A Kromasil C18(250 mm ×4. 6 mm, 5 μm) column was adopted. The mobile phase consisted of acetonitrile-0. 085 % phos-phoric acid(17 : 83)with the flow rate of 1.0 mL/min. The column temperature was at 35 ℃ and detection wavelength was set at 316 nm. Results A good linearity of ferulic acid was in the range of 0. 0252μg to 0. 504μg and r=0. 999 9. The average recovery was 100. 15 % and RSD=1.69 %. Conclusion The method is simple and rapid, and it is suitable for the determi-nation of ferulic acid in Xuefu Zhuyu Decoction.
ABSTRACT
In continuation of our investigation, a new diterpene, rabdonervosin C (Ⅰ), was isolat-ed from the leaves and stems of Rabdosia nervosa (Hemsl.) C. Y. Wu et H. W. Li. Its structure wasidentified as lα, 15β, -dihydroxy-6β-ethoxy-6, 7-B-seco-ent-kaur-16-en-6, 20-epoxy-7, 20-δ-olide on thebasis of spectroscopic (MS, IR, 1HNMR, 13CNMR, DEPT and 2D-NMR) data and in comparison with theknown rabdonervosin A(Ⅲ) and B (Ⅱ).