ABSTRACT
OBJECTIVE:To study induction effect of euphornin on the apoptosis of cervical cancer Hela cells and its mechanism. METHODS:The cervical cancer Hela cells were divided into blank control group,cisplatin group(positive control, 10 mg/L) and euphornin low-dose,medium-dose and high-dose groups (50,100,200 mg/L). They were treated with relevant medicine. The inhibitory effect of Hela cells proliferation was tested by MTT assay after 24,48,72 h of medicine treatment. The apoptotic rate of Hela cells was measured by flow cytometry after 48 h of medicine treatment. Morphology of nucleus was detected by Hoechst 33258 staining. The protein expression of Cyt-C,Bcl-2,Bax,Caspase-3,Caspase-8,Caspase-9 and Caspase-10 were detected by Western blot assay. RESULTS:Compared with blank control group,inhibitory rate of cell proliferation and cell apoptosis rate were increased significantly in cisplatin group and euphornin groups(P＜0.05 or P＜0.01),and obvious staining, deformation,shrinking,fragmentation or apoptotic bodies was found in nucleus. Compared with blank control group,the protein expression levels of Cyt-C,Caspase-8 and Caspase-9 in euphornin low-dose,medium-dose and high-dose groups were increased significantly,while the protein expression level of Bcl-2 and Bcl-2/Bax ratio were decreased significantly(P＜0.05 or P＜0.01);the protein expression levels of Bax,Caspase-3 and Caspase-10 in euphornin medium-dose and high-dose groups were increased significantly(P＜0.05 or P＜0.01). CONCLUSIONS:Euphornin can significantly inhibit the proliferation of Hela cell and promote cell apoptosis,the effect of which will be achieved by activating the Caspase-dependent mitochondrion apoptosis pathway.
ABSTRACT
Objective To analyze the diagnostic value of human leukocyte antigen(HLA)-B27 expression for ankylosing spondylitis.Methods 100 patients with ankylosing spondylitis were selected as the study group. 100 healthy people were selected as the control group.HLA -B27 beads enzyme immunoassay reagent kits was used to implement qualitative detection of fresh whole blood in the study group,and compared HLA -B27 positive rates of the two groups,as well as the diagnosis value of HLA -B27 antigen expression.Results The HLA -B27 positive rate of the study group was 73.00%,which was higher than 9.00% of the control group,the difference was statistically significant (χ2 =84.66,P <0.01).The diagnosis rate of the male patients was 90.91%,which was higher than 72.41% of the female patients,the difference was statistically significant (χ2 =4.35,P <0.05 ).Conclusion HLA -B27 antigen has a high value for clinical diagnosis of ankylosing spondylitis,which is worthy of promotion.