Study of gene correlation between endothelial growth factor family in mouse eye / 中华眼底病杂志

Objective:To compare the function and action pathways of VEGFA, VEGFB and VEGFC in VEGF family of mouse eye.Methods:Using the BXD mouse gene data in Genenetwork database as template to compare and study the similarities and differences of VEGFA, VEGFB and VEGFC molecular pathways or potential functions in the whole genome expression spectrum of BXD recombinant mouse inbred line population, with multiple analytical methods and statistical strategies were used, such as gene expression level, target genes comparison, top genes comparison associated to target genes, expression Quantitative Trait Loci (eQTL).Results:Matrix comparison showed strong positive correlation between two probes of VEGFC ( r=0.732, P<0.01), weak correlation between VEGFA 1420909 and VEGFC 1440739, VEGFA 1451959 and VEGFC 1451803, VEGFC 1419417959 and VEGFC 1439766, VEGFC 1451803 and VEGFC 1439766 ( P<0.05); there was no correlation between VEGFA 1420909 and four other genes except VEGFC 1440739, VEGFA 1451959 and VEGFC 1440739, VEGFB 1451803 and VEGFA 1420909/VEGFC 1419417/VEGFC 1440739 ( P >0.05). In the comparative analysis of the relevant Top 50 genes of each VEGF gene, most of the genes in BXD mouse were not significantly correlated with VEGFA, VEGFB and VEGFC except for the weak association of individual related genes. The results of eQTL analysis showed that each probe of VEGF gene was located on different chromosomes. Conclusions:The expression levels and positive and negative correlations of VEGFA, VEGFB and VEGFC were different in the VEGF family of mouse eye, suggesting that these genes may play their role through different pathways.

Prevalence and risk factors of cardiac events in different ethnic groups in Xinjiang region / 中华健康管理学杂志

Objective:To explore the prevalence and risk factors of cardiac events in different ethnic groups in Xinjiang region.Methods:This retrospective cohort study was based on big data from the health checkup population. A total of 7 899 cases were included from the Physical Examination Center of Xinjiang Uygur Autonomous Region People′s Hospital form 2015 January 1 to December 31, 2017.The population were divided into Uyghur group (2 630 cases), Kazak group (2 636 cases), and the Han nationality group (2 633 cases). Telephone follow-up was conducted once a month after the health checkup, the preset follow-up time for all personnel was 2 years, with the occurrence of cardiac events as the end point. Once cardiac events occurred, the follow-up would be stopped. The risk factors of cardiac events in different ethnic groups were evaluated by statistical analysis.Results:The median follow-up time of the 7 899 included healthy examinees was 1.27 years, and 200 cases of cardiac events occurred, with an incidence rate of 2.53%. The values of body mass index (BMI), the levels of triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) of Uyghur and Kazak were higher than those of Han (all P<0.05). The cardiac events in Uyghur, Kazak and Han group were 75 cases (2.85%), 85 cases (3.22%) and 40 cases (1.52%). There was no significantly statistical difference between Uyghur group and Kazak group in the incidence of cardiac events, while it was significantly lower in the Han group than the other two groups (both P<0.05). Univariate analysis showed that BMI, TC, TG, HDL-C and LDL-C were the risk factors of cardiac events; multivariate Cox regression analysis showed that ethnic groups ( HR=4.34, 95% CI: 1.14―8.13); HDL-C ( HR=3.32, 95% CI: 1.89―5.74) and LDL-C ( HR=2.47, 95% CI: 1.21―7.45) were independent risk factors for cardiac events. Conclusions:Ethnic factor is one of the independent risk factors for the occurrence of cardiac events in Xinjiang, and Uyghur and Kazak have a higher incidence of cardiac events. HDL-C and LDL-C are also important risk factors for cardiac events.

Measure the Parameters of Morphology of Pathology by Image Processing Toolbox / 中国医学物理学杂志

Objective: Digital images processing is widely applied in the fields of biology and medicine, and some achievements have been gained by this technique. But there is few reports on measure the parameters of morphology of pathology from the patients which were threated by ovulation induction, by computer processing techniques. The work shows it has significant value in clinical diagnosis and reproductive medicine at present and in the future. Methods: Write the programmeces by Image Processing Toolbox in MATLAB. The original pathology images are preprocessed by 4 steps: step 1: read imagejstep 2: imopen the curve background brightness; step 3: adjust the background; step 4: adjust the contrast. Then segmentation image by 4 steps; step 1: read image; step 2: estimation the image; step 3: remove the noise; step 4: find the boundaries. The end, measure the parameters of morphology. Results: The pathology was studied percase for their area, perimeter, maximum diameter, equivalent diameter and metric. Conclusions: Image Processing Toolbox is useful to measure the parameters of morphology of pathology.

Rapid tissue microarray assay of p16 protein expression for different stage nasopharyngeal carcinoma / 中华病理学杂志

<p><b>OBJECTIVE</b>To effectively screen p16 protein expression of different clinical stage nasopharyngeal carcinoma (NPC) by constructing and applying high-throughput tissue microarray/tissue chip.</p><p><b>METHODS</b>A series of tissue chips were prepared by using tissue arrayer with samples from different clinical stage NPC tumors and noncancerous nasopharynx tissue. Specimens from 259 cases of nasopharyngeal lesions were detected immunohistochemically on a tissue chip for p16 protein expression and the correlation of p16 protein expression to clinical stage of NPC was analyzed statistically.</p><p><b>RESULTS</b>p16 protein expression was detected in all 18 histologically normal nasopharyngeal epithelia. No p16 protein was detected in 3 of 3 (100%) stage I NPC, 38 of 44 (86.3%) stage II NPC, 59 of 68 (86.8%) stage III NPC, 23 of 28 (82.1%) stage IV NPC, 87 of 98 (88.8%) unclear stage NPC. The efficiency of p16 protein expression in NPC tissues was significantly lower than that in normal nasopharyngeal epithelia (chi(2) = 82.58, P < 0.001), and there was no apparent relationship between p16 protein expression and clinical stages (chi(2) = 0.09, P = 0.769).</p><p><b>CONCLUSIONS</b>The frequent deletion of p16 protein in NPC suggests that p16 gene has an important role in the development and progression of NPC. The consistency of p16 protein deletion in different stages of NPC suggests that the deletion of p16 protein is an early event in the development of NPC, and it is feasible to utilize tissue microarray for a rapid, economic and accurate screening of clinical tissue specimens on a large scale.</p>

Evidence for high-frequent deletion of p53 gene in primary hepatocellular carcinoma by interphase dual fluorescence in situ hybridization / 中国病理生理杂志

AIM: To investigate the frequency and pattern of deletion of p53 gene in primary hepatocellular carcinoma (HCC) and its clinical significance. METHODS: The interphase dual fluorescence in situ hybridization(FISH) technique was applied to detect loss of p53 gene in HCCs. RESULTS: The deletion of p53 gene was found in 68.0% of HCCs whereas no loss of p53 gene was detected in 40 mated normal liver specimens. Loss of p53 gene was closely related to tumor size and serum ?-fetoprotein(AFP) level in HCC patients ( P 0.05). The 2-year survival rate of postoperative HCC patients was significantly lower in the HCC cases with p53 gene deletion (25.6%) than those without p53 gene loss (69.6%) ( ? 2=11.463, P