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Objective To investigate the effect of flipped classroom based on WeChat and mi-crolecture in medical chemistry experiment course in the context of "internet". Methods The classes were randomly divided into 2 groups, experimental group (flipped classroom teaching, n=97) and the control group (traditional teaching, n=98). Comparison of the chemistry experiment test results were performed with the use of t test between the two teaching groups at the end of the semester to evaluate the experimental teaching method. All statistical processing and analyses were performed with SPSS software (version 12.0). Results The chemistry experiment test score of the experimental group was higher than that of the control group [(77.84±8.22) vs. (73.43±10.14), t=3.341, P=0.008), and the difference was statistically significant. The results of the questionnaire showed that the students in the experimental group generally consider that flipped classroom teaching is better than the traditional teaching in terms of the cultivation of comprehen-sive quality and the teaching effect. Conclusion In the context of "internet", flipped classroom teaching with WeChat microlecture can better mobilize the enthusiasm of students to learn and participate in medical chemistry experiment course, which has been welcomed by students and further suggest good application prospects.
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Objective To explore an optimal method of producing STZ-induced type 1 diabetic KM mice model by investigating the molded rate of single high dose and multiple low dose of STZ injection.Methods Sixty KM mice were randomly divided into 4 groups(n=15), two control groups and two model groups.In the two control groups, one was blank control and the other was negative control.Mice in the blank control group treated with no injection, but mice in the negative control group were treated with injection of citric acid salt buffer.For the two model groups, one was single high-dose group, 150 mg/kg STZ was injected only once.The other was multiple low-dose group, 50 mg/kg STZ was injected for 5 consecutive days.After the last injection, daily food and water intake were tested, blood glucose level and body weight were examined once a week for 4 consecutive weeks. Results Mice in the two model groups showed typical features of diabetes.The blood glucose levels in the two model groups were significantly higher than in the two control groups ( P <0.05 ) .For the two model groups, the molded rate of 150 mg/kg and 50 mg/kg group were 60% and 53.33%respectively at 1st week, but at the 4th week, they were 60% and 80% respectively.The mortality of these two groups at the 4th week was 33.33% and 20% respectively.Moreover, the blood glucose level in multiple low-dose group increased stably from the 2nd week to the 4th week.Conclusion The multiple low-dose STZ injection (50 mg/kg for 5 consecutive days) is an optimal method for producing KM mice model of type 1 diabetes mellitus.
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Objective To provide evidences for rational use of antibiotics in treatment of hospital-acquired urinary tract infection.Methods The bacteria isolated from mid-stream urine samples were collected for susceptibility test(MIC)using international standard plate dilution method.According to breakpoints defined in CILS guidelines(2009),each strain of bacteria was determine for susceptibility to antimicrobial agents,and calculated for rates of resistance(R%),intermediate(I%)and susceptibility(R%)to compounds tested.Results 552 strains of bacteria were collected,including 432 strains(78.3%)of Gram-negative bacilli and 120(21.7%)of Gram-positive bacilli;Escherichia coli was one of the most common bacteria in the urinary tract infection(55.3 %),followed by Enterococcus(17.4%).The results of antibiotic susceptibility test in vitro showed that Enterobacteriaceae was 100% susceptible to imipenem;Gram-positive cocci were sensitive most to glycopeptide antibiotics.Conclusion Clinicians should pay attention to the species of pathogenic bacteria causing urinary tract infection and their susceptibility to clinically common used antibiotics for reasonable use of drugs.
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Objective\ To evaluate the possibility of predicting renal function from creatinine clearance calculated by lean body weight(LBWCcr).Methods\ 81 patients of renal disease were selected.The ratio between 24-hour urinary creatinine excretion(24hUcr)and lean body weight(LBW)was evaluated in 51 patients.The other 30 patients was determined DTPA-GFR,LBWCcr and 4hCcr.DTPA-GFR was used as criteria.LBWCcr and 4hCcr were compared with it in order to validate the feasibility of LBWCcr and 4hCcr.Results\ There was no statistical significance among the three methods,P=0 56 The correlation coefficient between LBWCcr and 4hCcr with DTPA-GFR was 0 89 and 0 86,respectively.The analysis of linearity regression showed that the trend of LBWCcr was better than 4hCcrmin in predicting renal function.In fatty group and renal failure group the results were similar.Conclusion\ LBWCcr can estimate the renal function accurately,simply and rapidly.
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Objective To investigate the feasibility whether clearance of iohexol can be a reliable, sensitive and safe method for the determination of GFR. Methods The GFR of 19 patients with different renal functions were examined using clearance of 99m Tc-DTPA and clearance of iohexol. Then the correlation of them was analyzed. Serum and urinary iohexol was determined by X-ray fluorescence analysis. Results, These two methods were significantly correlated (r = 0. 98). Conclusion Clearance of iohexol is a safe, comfident, no-radioactivity method for the clinical practice of GFR determination.