ABSTRACT
Objective To investigate the implementation effect of group health education mode in cataract department. Methods Through the establishment of clinical health education path and repeatedly broadcast in group health education after surgery for 1200 cases of cataract patients,effect of health education and satisfaction on surgery were assessed and surveyed. Results Knowledge of health education was known by about 88.5% of patients or their family;satisfaction with nurse care was up to 95.0%; health education time of the nurses was reduced but their work efficiency was improved. Conclusions Implementation of group health education mode and clinical health education path could satisfy patients' demands of health education in large-scale cataract surgery and ensure the quality of health education.
ABSTRACT
Apolipoprotein E (ApoE) was purified from human serum and used as the antigen to immunize BALB/C mice. The splenic cells of the mice fused with NS-1 mouse myeloma cells. Three hybridoma cell lines(E3C3, E6C3 and E4C2)secreting anti-human ApoE McAb were established. The immunological property of the McAb was studied. The ascites fluids titer were 8?10~(-5)2?10~(-6). The McAbs did not cross react with the other apolipoprotins. ApoE was purified by immunoaffinity chromatography prepared by using the ApoE McAb. McAbs recognized two distinct respective epitopcs on ApoE. The subclass of immunoglabulin of three McAbs were IgG_1. A double McAb sandwich EL1SA was developed to evaluate ApoE levels in normal and hyperlipidemia human serum.