ABSTRACT
BACKGROUND: Runx2 plays a central role in osteogenic differentiation, which is of important significance in new bone formation.OBJECTIVE: To observe the effect of Runx2 over-expression on osteogenic differentiation of human umbilical cordblood mesenchymal stem cells.METHODS: Runx2 was generated by RT-PCR and the recombinant adenovirus (pAd-Runx2) was constructed. The viraltiter was determined by air dilution method. After being transfected into human umbilical cord blood mesenchymal stemcells, the green fluorescence was observed under fluorescence microscope. Real-time fluorescent quantitative PCR andwestern blot were used to detect mRNA expression changes of osteogenesis related genes, Runx2, OCN, BMP-2, ALP,in human umbilical cord blood mesenchymal stem cells at 1, 3, 7 and 14 days after transfection.RESULTS AND CONCLUSION: The recombinant adenovirus was successfully constructed and the titer was1.7×1010 pfu/L. After infection by pAd-Runx2, human umbilical cord blood mesenchymal stem cells expressed greenfluorescence protein clearly under the fluorescence microscope. Cells in the transfected group differentiated intoosteoblast-like cells, and those in the control group stayed the same as pre-infected. The expression of Runx2, OCN,BMP-2 and ALP in the transfected group increased over time to some extent, but these changes were not detected in thecontrol group. These findings indicate that Runx2 over-expression can promote the osteogenic differentiation of humanumbilical cord blood mesenchymal stem cells.
ABSTRACT
Objective To investigate the causes and prevetion of urethral stricture after transurethral resection of the prostate(TURP). Methods A retrospective analysis was carried out in 945 cases,who were treated by TURP in our hospital during February 2002-February 2008.Among the cases,21 patients(aged 62 to 84 years with a mean of 72.4 years) developed urethral stricture in 3 weeks to 24 months after the operation.In the cases,anterior urethral stricture was detected in 13 patients(61.9%),posterior urethral stricture was found in 5(23.8%),and bladder neck contracture was diagnosed in 3(14.3%).Results The pateints were cured by simple urethral dilation(13,61.9%),urethroplasty(2,9.5%),urethroplasty plus urethral dilation(2,9.5%),transurethral resection(1,4.8%),or electroresection of the bladder neck(3,14.3%).The patients were followed up for 6 to 36 months(mean,13 months),during which,all the 21 cases recovered without recurrence.The rate of urethral stricture after TURP was 3.7%(18/483) during February 2002 to August 2005,which was significantly higher than that between September 2005 and February 2008(0.6%,3/462;?2=10.292,P=0.001).Conclusions Unskilled operation and short indwelling time of urethral catheter after the surgery are the major reasons for urethral stricture after TURP.Regular follow-up and early treatment are the keys to cure of the disease.
ABSTRACT
Object To determine the content of resveratrol glucoside and resveratrol in radix and rhizome of Polygonum cuspidatum Sieb. et Zucc. yielded in Hanzhong Region. Methods The HPLC method was used to assay resveratrol glucoside and resveratrol directly, using Eclipse XDB C 8 column and acetonitrile water as the mobile phase, the UV detection wavelength was 303 nm, with a flow rate of 1 mL/min. Results The content of resveratrol glucoside was 2 5% and resveratrol was 0 43% in the radix and rhizome. Conclusion The content of resveratrol glucoside is higher than that in the former refernece. The content of resveratrol in the radix and rhizome of P. cuspidatum in Hanzhong Region is first reported.