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ABSTRACT Introduction: Some studies point out that high-intensity intermittent training exercises combined with drugs may improve the endurance of patients with type 2 diabetes. However, the information concerning blood glucose control still needs to be better evidenced. Objective: Explore further the effect of intermittent high-intensity exercise combined with drugs on blood glucose control in patients with type 2 diabetes. Methods: Through a control experiment, 100 patients were selected from volunteers, and divided equally into two groups for this experiment. A protocol with high-intensity intermittent exercise for intervention associated with drug treatment was added to the experimental group, while the control received standard drug treatment. Results: The results of each index in the experimental group were: TC pre 4.80±1.00, post 4.56±0.78; TG pre 1.77±1.15, post 1.49±1.16; LDL pre 2.94±0.83, post 2.51±0.73. The experimental results proved that all indices in the experimental group were improved, but the changes in the control group were not evidenced. Conclusion: Intermittent high-intensity exercise combined with drugs positively affected blood glucose control in patients with type 2 diabetes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: Alguns estudos apontam que exercícios com treinamento intermitente de alta intensidade combinados aos fármacos possam melhorar a resistência dos pacientes com diabetes tipo 2. No entanto, as informações relativas ao controle da glicemia ainda são pouco evidentes. Objetivo: Explorar melhor o efeito do exercício intermitente de alta intensidade combinado com fármacos no controle glicêmico sanguíneo em pacientes com diabetes tipo 2. Métodos: Através de um experimento de controle, 100 pacientes foram selecionados entre os voluntários, divididos igualmente em dois grupos para este experimento. Ao grupo experimental foi adicionado um protocolo com exercício intermitente de alta intensidade para intervenção associado ao tratamento medicamentoso, enquanto o controle recebeu tratamento medicamentoso padrão. Resultados: Os resultados de cada índice no grupo experimental foram: TC pré 4,80±1,00, pós 4,56±0,78; TG pré 1,77±1,15, pós 1,49±1,16; LDL pré 2,94±0,83, pós 2,51±0,73. A partir dos resultados experimentais, comprovou-se que todos os índices no grupo experimental foram aprimorados, porém as mudanças no grupo de controle não foram evidenciadas. Conclusão: O exercício intermitente de alta intensidade combinado com fármacos apresentaram um efeito positivo no controle da glicose sanguínea em pacientes com diabetes tipo 2. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: Algunos estudios señalan que los ejercicios de entrenamiento intermitente de alta intensidad combinados con fármacos pueden mejorar la resistencia de los pacientes con diabetes de tipo 2. Sin embargo, la información relativa al control de la glucemia sigue siendo escasa. Objetivo: Profundizar en el efecto del ejercicio intermitente de alta intensidad combinado con fármacos sobre el control de la glucemia en pacientes con diabetes tipo 2. Métodos: Mediante un experimento de control, se seleccionaron 100 pacientes voluntarios, divididos equitativamente en dos grupos para este experimento. Al grupo experimental se le añadió un protocolo con ejercicio intermitente de alta intensidad para la intervención asociado al tratamiento farmacológico, mientras que el control recibió el tratamiento farmacológico estándar. Resultados: Los resultados de cada índice en el grupo experimental fueron: TC pre 4,80±1,00, post 4,56±0,78; TG pre 1,77±1,15, post 1,49±1,16; LDL pre 2,94±0,83, post 2,51±0,73. A partir de los resultados experimentales, se comprobó que todos los índices del grupo experimental mejoraron, pero no se evidenciaron cambios en el grupo de control. Conclusión: El ejercicio intermitente de alta intensidad combinado con fármacos tuvo un efecto positivo sobre el control de la glucemia en pacientes con diabetes tipo 2. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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Since metabolic process differs between humans and mice, studies were performed in hamsters, which are generally considered to be a more appropriate animal model for studies of obesity-related metabolic disorders. The modulation of gut microbiota, bile acids and the farnesoid X receptor (FXR) axis is correlated with obesity-induced insulin resistance and hepatic steatosis in mice. However, the interactions among the gut microbiota, bile acids and FXR in metabolic disorders remained largely unexplored in hamsters. In the current study, hamsters fed a 60% high-fat diet (HFD) were administered vehicle or an antibiotic cocktail by gavage twice a week for four weeks. Antibiotic treatment alleviated HFD-induced glucose intolerance, hepatic steatosis and inflammation accompanied with decreased hepatic lipogenesis and elevated thermogenesis in subcutaneous white adipose tissue (sWAT). In the livers of antibiotic-treated hamsters, cytochrome P450 family 7 subfamily B member 1 (CYP7B1) in the alternative bile acid synthesis pathway was upregulated, contributing to a more hydrophilic bile acid profile with increased tauro--muricholic acid (TMCA). The intestinal FXR signaling was suppressed but remained unchanged in the liver. This study is of potential translational significance in determining the role of gut microbiota-mediated bile acid metabolism in modulating diet-induced glucose intolerance and hepatic steatosis in the hamster.
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Objective To analyze the effect of the identification and evaluation of Escherichia (E.) coli and Shigella,based on the upstream flanking sequences of CRISPR1.Methods Both CRISPR and cas sequences were obtained through the BLAST with repeating sequences against the publicly complete genome in GenBank that related to E.coli and Shigella.Clustal X was used to perform multi-sequences alignment of the flanking sequences.PCR method was used to amplify the upstream flanking sequences of CRISPR1 in order to appraise the effect of identification and evaluation of upstream flanking sequences on E.coli and Shigella,which were based on the upstream flanking sequences of CRISPR1.Results The results showed that 73.4％ of the strains containing the I-E CRISPR/Cas that belonged to the phylogroups A,B1,D while 8.4％ strains carried the I-F CRISPR/Cas.Another 17.2％ of the strains owned CRISPR3-4 (non-CRISPR/Cas) only belonged to the phylogroups B2.All the Shigella strains carried I-E CRISPR/Cas.More than 99％ of similarity the CRISPR1 upstream-flanking sequences was seen in E.coli (except B2) and Shigella and E.coli (B2).Both sensitivity and specificity were greater than 91％ after PCR amplification in the region to identify the E.coli and Shigella.Conclusion The upstream of CRISPR1 could achieve a preliminary identification effect on E.coli and Shigella.
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Objective To analyze the effect of the identification and evaluation of Escherichia (E.) coli and Shigella,based on the upstream flanking sequences of CRISPR1.Methods Both CRISPR and cas sequences were obtained through the BLAST with repeating sequences against the publicly complete genome in GenBank that related to E.coli and Shigella.Clustal X was used to perform multi-sequences alignment of the flanking sequences.PCR method was used to amplify the upstream flanking sequences of CRISPR1 in order to appraise the effect of identification and evaluation of upstream flanking sequences on E.coli and Shigella,which were based on the upstream flanking sequences of CRISPR1.Results The results showed that 73.4％ of the strains containing the I-E CRISPR/Cas that belonged to the phylogroups A,B1,D while 8.4％ strains carried the I-F CRISPR/Cas.Another 17.2％ of the strains owned CRISPR3-4 (non-CRISPR/Cas) only belonged to the phylogroups B2.All the Shigella strains carried I-E CRISPR/Cas.More than 99％ of similarity the CRISPR1 upstream-flanking sequences was seen in E.coli (except B2) and Shigella and E.coli (B2).Both sensitivity and specificity were greater than 91％ after PCR amplification in the region to identify the E.coli and Shigella.Conclusion The upstream of CRISPR1 could achieve a preliminary identification effect on E.coli and Shigella.
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Objective To explore the relationship between social anxiety and acceptance of disability in breast cancer patients with mastectomy. Methods Totally 325 patients with breast cancer were investigated with general information questionnaire, Interaction Anxiety Scale and Acceptance of Disability Scale.Results The total score of Interaction Anxiety Scale was(40.01±9.38)points.The total score of Acceptance of Disability Scale was (78.02 ± 11.61) points. One-way ANOVA showed that age, education level, marital status, economic level, whether the spouse care about the appearance or not, therapy types affected social anxiety significantly(t/F=-4.696-35.694,all P<0.01).Significantly negative correlation was found between social anxiety and acceptance of disability (r =-0.469--0.371, P<0.01). Multiple regression analysis showed that acceptance of disability,age,whether the spouse care about the appearance or not, therapy types were influencing factors of social anxiety. Conclusions Nurses and doctors should explore effective psychological intervention mode to rebuild the patient′s self-confidence and return to normal social interaction in order to improve the acceptance of disability.
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Objective To investigate the effect of interventions based on Information-Motivation-Behavioral skills on the preoperative anxiety in patients with gynecological malignancies. Methods A total of 64 patients with malignant tumor were divided into two groups by random digits table method with 32 cases each. The patients in the two groups received routine nursing. In addition, interventions based on Information-Motivation-Behavioral skills were provided in the intervention group. All patients were investigated by the following indexes such as the Self-Rating Anxiety Scale (SAS), sleep quality scale, blood pressure and heart rate before and after the intervention. Results After the intervention, the SAS scores in the intervention group was (49.47 ± 3.81) points, sleep quality score was (3.66 ± 0.97) points, and systolic blood pressure and heart rate were (128.56±5.93) mmHg (1 mmHg=0.133 kPa), (75.09 ± 3.78) beats/min, which were lower than those in the control group (57.38 ± 3.75) points, (5.50 ± 1.50) points, (134.97 ± 7.19) mmHg, (81.34 ± 4.88) beats/min, the differences were statistically significant (t=-8.350--3.887, P<0.05). Conclusions The intervention focused on Information-Motivation-Behavioral Skills can help patients to relieve anxiety, reduce stress response, improve quality of sleep.
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Objective To investigate the association between phage-mediated shiga toxin and molecular distribution of CRISPR in Escherichia (E.) coli O26:H11 or NM.Methods A total of 135 E.coli O26:H11 or NM strains were collected from NCBI database.Software CRT and CRISPR Finder were used to extract CRISPR and Excel was used to assign the spacer of unique number and type CRISPR.And the relationship between CRISPR and stx phage was analyzed.Results All the 135 E.coli O26:H11 or NM strains had the CRISPR.For CRISPRI,CRISPR2.1,CRISPR2.2 and CRISPR3-4,19,22,1 and 1 subtypes were found,respectively.According to the four CRISPR sites,the strains could be divided into 40 subtypes.Stx-phage was only observed in the group C of CRISPR.Compared with E.coli of stx-phage negative,E.coli with stx-phage harbored more spacers.Conclusions CRISPR loci was extensively existed in E.coli O26:H11 or NM,and many subtypes were found in these strains.The presence of stx-phage was related to the molecular distribution of CRISPR in E.coli O26:H11 or NM.CRISPR might be a valuable biomarker to identify strains with high virulent potential.
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Objective To investigate the association between phage-mediated shiga toxin and molecular distribution of CRISPR in Escherichia (E.) coli O26:H11 or NM.Methods A total of 135 E.coli O26:H11 or NM strains were collected from NCBI database.Software CRT and CRISPR Finder were used to extract CRISPR and Excel was used to assign the spacer of unique number and type CRISPR.And the relationship between CRISPR and stx phage was analyzed.Results All the 135 E.coli O26:H11 or NM strains had the CRISPR.For CRISPRI,CRISPR2.1,CRISPR2.2 and CRISPR3-4,19,22,1 and 1 subtypes were found,respectively.According to the four CRISPR sites,the strains could be divided into 40 subtypes.Stx-phage was only observed in the group C of CRISPR.Compared with E.coli of stx-phage negative,E.coli with stx-phage harbored more spacers.Conclusions CRISPR loci was extensively existed in E.coli O26:H11 or NM,and many subtypes were found in these strains.The presence of stx-phage was related to the molecular distribution of CRISPR in E.coli O26:H11 or NM.CRISPR might be a valuable biomarker to identify strains with high virulent potential.
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Objective To evaluate the effect of written expression on perceived stress,anxiety and depression in patients with gynecologic malignant tumor during chemotherapy. Methods From June 2016 to December 2016,90 chemotherapy patients with gynecologic malignant tumor were extracted from North China University of Science and Technology and divided into intervention group and control group by random digits table method,45 cases in each group.The control group received routine psychological intervention and while the intervention group received written expression lasting for 4 weeks in addition. Before and after the intervention,all patients were measured using the Chinese Version Perceived Stress Scale(CPSS), Self-Rating Anxiety Scale(SAS) and Self-Rating depression Scale(SDS). Results The intervention group showed a significant decrease in CPSS(32.12 ± 3.75) points, SAS(43.67 ± 5.23) points, SDS(46.18±4.37)points,compared with the control group(37.26±4.42),(50.88±3.15),(52.47±3.60)points, the difference was significant(t=-5.782,-7.667,-7.230,all P<0.05). Conclusions Written expression can effectively reduce perceived stress, anxiety and depression in patients with gynecologic malignant tumor undergoing chemotherapy.
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Salvia miltiorrhiza bunge (Dan shen in Chinese)is extracted from dried roots and rhizomes of Labiatae Salvia.Tan-shinones are the main lipid-soluble components in Salvia,each has specific pharmacological activity.This review focuses on the research progress of Tanshinones on cardiovascular diseases,an-ti-inflammatory and immunomodulatory effects, anti-tumor effect,hepatocyte protection and neuroprotective effects.Thera-peutic effects and mechanisms of Tanshinones on diverse disea-ses are summarized,pharmacokinetics and pharmaceutic evalua-tion were concluded.This review provides a global understand-ing about Tanshinones as a class of effective and promising can-didates for further studies,and lays a foundation for developing new Tanshinone-based agents according to the characteristics of Tanshinones.
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BACKGROUND: Eye acupuncture therapy is a technique used to adjust qi-blood circulation, relax muscles and tendons, and activate col aterals by acupuncture at the acupoints around the eye bal s and in the orbital border. This therapy has been widely used in the clinic because it exhibits remarkable therapeutic effects on many ischemic cerebrovascular diseases. However, the precise mechanism behind this therapy remains poorly understood. Neurotrophic factors are a protein family including neurotrophic factors and brain-derived neurotrophic factors that can regulate neuronal survival, development and functioning. OBJECTIVE: To investigate the effects of eye acupuncture therapy on neurological function and nerve growth factor and brain-derived neurotrophic factor expression in the brain tissue of rat models of cerebral ischemia/reperfusion injury. METHODS: Fifty-four male Sprague-Dawley rats were randomly divided into three groups with 18 rats per group: sham-operated, model and eye acupuncture therapy groups. Rat models of middle cerebral artery occlusion were established by the intraluminal suture method in the model and eye acupuncture therapy groups. Eye acupuncture was performed at the fol owing acupoints liver area, upper-jiao area, lower-jiao area and kidney area located at the internal orbital margin at 2 hours after cerebral ischemia/reperfusion injury. Rat neurological function was evaluated at 3, 7, and 14 days after injury. Nerve growth factor and brain-derived neurotrophic factor expression in the rat brain tissue was detected using immunohistochemical staining method at 1 and 2 weeks after treatment. Cerebral infarct size was determined using TTC staining at 2 weeks after treatment. RESULTS AND CONCLUSION: (1) At 1 and 2 weeks after injury, nerve growth factor and brain-derived neurotrophic factor expression was significantly greater in the eye acupuncture therapy group than in the model group (P < 0.05), but nerve growth factor and brain-derived neurotrophic factor expression in the eye acupuncture therapy group was decreased at 2 weeks after injury compared to that at 1 week after injury. (2) At 7 and 14 days after treatment, neurological function scores in the eye acupuncture therapy group were significantly lowered, and there was significant difference between eye acupuncture therapy and model groups (P < 0.05), but they were significantly higher than those in the sham-operated group (P< 0.05). (3) At 2 weeks after treatment, cerebral infarct size was significantly greater in the eye acupuncture therapy and model groups than in the sham-operated group (P < 0.01), and it was significantly smal er in the eye acupuncture therapy group than in the model group (P < 0.05). (4)These results indicate that eye acupuncture therapy shows neuroprotective effects on ischemic cerebral injury by increasing nerve growth factor and brain-derived neurotrophic factor expression, improving neurological function, and reducing cerebral infarct size.
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Objective:To observe the changes of TLR4 and IL-6 expression in rats hippocampus CA1 region in remote ischemic postconditioning(RIP) and explore its significance.Methods: All the 72 male SD rats were divided into Sham group,Contrast group and RIP group randomly.Each group was divided into 4 time points:12h,24 h,48 h and 72 h group.There were 6 rats in each group.Use the cerebral ischemia-reperfusion model which was established with modified Longa method as the contrast group.The method of RIP was to Fasten rats Posterior limbs by a tourniquet for 30 min immediately,then relax them for 30 min, repeat 3 times.To observe the pathological variation of hippocampus CA1 region by HE dyeing;to test the expression of TLR4 and IL-6 by immunohistochemical staining.Results: Compared to contrast group, neuronal loss and swelling reduced significantly in RIP group.Compared to sham group, the TLR4 and IL-6 expression in contrast group and RIP group increased significantly ( P<0.05 ) .Compared to contrast group,the TLR4 and IL-6 expression in RIP group reduced significantly(P<0.05).Conclusion:RIP dose have protective effect on cerebral ischemia.The effect may be associated with the inhibition of TLR4 and IL-6 expression.
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Objective To investigate the risk factors affecting prognosis in elderly patients with septic shock and explore the effective treatment. Methods A retrospective study of 147 patients with septic shock from the Affiliated Hospital of Hebei United University and Affiliated People's Hospital from Jan. 2011to Jan. 2013 was conducted. Early fluid loading was defined as the administration with an initial fluid bolus within 6 h in ICU. Conservative fluid management was defined as negative fluid balance measured in at least 1 d during the first 3 d as the case group,while the daily fluid balance and cumulative daily fluid balance of the first 3 d was as the control group. The effects of early fluid management on prognosis of elderly patients septic with shock combining the living index within 7 d in ICU were recorded. Results In the case group with 7 d ICU,21. 4%(15 / 70)patients were in progressed and 37. 1%(26 / 70)patients were dead,while 25. 9%(20 / 77)patients were in progressed and 59. 7%(46 / 77)patients were dead in control group(χ2 = 13. 17,P = 0. 00). Univariate analysis showed that APACHEⅡ scores,SIRS score,organ failure,patients with chronic diseases related were associated with 7 d survival rate in elderly patients with septic shock. Multivariate Logistic regression analysis showed that higher APACHEⅡ scores and SIRS score,organ failure,patients with chronic diseases related (have)were the risk factors(OR = 1. 88,5. 34,1. 96 and 4. 76;P < 0. 05). The restrictive fluid management strategy have related with the septic shock patients in elderly that the OR of death rate of fluid management in elderly patients with septic shock was 0. 657(P = 0. 032). Conclusion The prognosis in elderly patients septic with shock is associated with APACHE Ⅱ scores,SIRS score,organ failure,patients with chronic diseases related. Early fluid management can improve to the prognosis of elderly patients with septic shock.
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Objective:To establish a detection method for residual toluene in diclofenac sodium raw material by GC. Methods:An HP-5 column (0. 53 mm × 30 m, 2. 65 μm) with nitrogen as the carrier gas was employed for the separation. The temperature of the column, injection port and FID detector was set at 50℃, 200℃ and 250℃, respectively. An internal standard method with isooctane as the internal standard was used to assay the residual amount after the sample was dissolved in DMSO. Results:The linear calibration curve was obtained over the range of 9. 27-111. 19μg·ml-1(r=0. 999 4). The mean recovery was 97. 27%(RSD=2. 80%,n=9). The LOD and LOQ was 0. 59μg·ml-1 and 1. 46μg·ml-1 , respectively. The residual contents of toluene in 3 batches of the products all met the relevant regulations. Conclusion:The established method is reliable and accurate, and can be used in the quality control of diclofenac sodium.
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Pancreatic cancer is a malignant with very poor prognosis.Although methods and technologies of diagnosis and treatment in connection with pancreatic cancer have made great progresses,the prognoses of patients with pancreatic cancer still have not a significant upgrade,which are closely related with the degree of malignancy of pancreatic cancer cells.Earlier studies have shown that normal pancreatic cells need to have a total of six capabilities,which are intimate connection with the degree of malignancy of pancreatic cancer cells,during the process of deterioration.A variety of immunohistochemical markers that correlate with prognosis of pancreatic cancer involve in the process of pancreatic cells obtaining these six capabilities.
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This paper introduces the ways to conduct the fixed denture teaching in prosthodontics with the multivariate teaching and learning mode,including information technology and PBL teaching,simulation model system,knowledge integration and the increasing of clinical practice.
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Combined with the partial least square regression, the oc tane number (RON), density, temperature evaporate (50%,90%), aromatics and olefi n concentration of gasoline were determined by the Fourier transform infrared spe ctroscopy at the wavenumber region of 1550～660cm－1. The experimenta l results showed that the actual values measured by the standard method were agre e with the prediction values by the proposed method.
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AIM: A methond was established for the determination of emodin and chrysophanol in Zhenhuang Powder (Margarita, Radix et Rhizoma Rhei, artificial Calculus Bovis, etc.). METHODS: RP HPLC was used for samples separated on a shim pack VP ODS column with the mobile phase of methanol-0.1% H 3PO 4(85∶15) and the detection wavelength was set at 430nm. RESULTS: The calibration curves were linear in the range of 0.01181～4.724?g( r =0.9994) for emodin and in the range of 0.01300～5.200?g( r =0.9999) for chrysophanol, respectively. The average recoveries were 99.0%( RSD =1.23%, n =5) and 100.5% ( RSD =1.06%, n =5), respectively. CONCLUSION: The method is simple, realizable and reproducible. It can be used effectively for the quality control of Zhenhuang Powder.