EFFECT OF OAT ?-GLUCAN ON COLON FLORA AND ITS FUNCTION IN MICE / 营养学报

Objective: To investigate the effect of oat?-glucan on colon flora and its function in mice. Method:126 Kunming mice(23-25g)were divided into seven groups (18 mice in each). The group 1 and 2, 3 and 4 ,5 and 6 were respectively administered oat?-glucan (OG2600 , OG340 and OG5) at dose of 0.25 and 0.5g/(kg bw?d)daily for 28 d. The control group received the equal volume of normal saline. Bifidobacterium , Lactobacillus and Enterobacillus were monitored in colon after oral administration of oat ?-glucan at 14 d, 28 d and 35 d (after terminating oat?-glucan admimistration for 1 w) . The contents of short chain fatty acids (SCFA) in colon were analyzed by GC and cell proliferation in colonic mucosa epithelium by FCM in each group. ResuIts:The numbers of Bifidobacterium , Lactobacillus compared with the control were significantly increased and Enterobacillus strains were decreased in experimental groups. Oat ?-glucan had the function of regulating intestinal flora and the effect was related to molecular weight and dosage. The contents of acetate, propionate, butyrate were higher in the group 2, 4 and 6 compared with the control (P

The protective effect of Vaccinium Bracteatum Thunb leaves and the extract against light injury of retina / 西安交通大学学报(医学版)

Objective To investigate the protective effect of the extract of Vaccinium Bracteatum Thunb(VBT) leaves against light injury of retina.Methods 20 Newzealand rabbits were divided into 4 groups: the normal control group,modelⅠgroup,modelⅡ group,and model Ⅲ group.The normal control group ate and drank freely.modelⅠgroup ate and drank freely but took in 50g/(kg?d) fresh VBT leaves daily for 4 weeks.model Ⅱgroup ate and drank freely,but took in 50g/(kg?d) old VBT leaves daily for 4 weeks.model Ⅲ group ate freely,but took in 50mg/(kg?d) extract of VBT leaves(resolved in water) daily for 4 weeks.All rabbits were exposed to strong light,meanwhile, the retinas were analyzed by electroretinogram(ERG) every week.Finally,all the rabbits were killed and the content of MDA and SOD in the retina were detected.Results In the control group,the latency time of the B wave increased and the amplitude of the B wave shortened,while in the model Ⅰ,Ⅱ and Ⅲ groups,the changes of B wave just opposite.The content of MDA in the model Ⅰ,Ⅱ,and Ⅲ groups was lower than that in the control group(P