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Objective To analyze the death cases of hospitalized children in Yuying Children's Hospital of Wenzhou Medical College from January 2004 to December 2013,and to detect the age distribution of deaths,the total cost and cost of different diseases,length of hospital stays,season distribution of deaths and mortality rates trends.Methods Five hundred and thirty-one death cases of 0-18 years old hospitalized children from 219 429 cases in Yuying Children's Hospital of Wenzhou Medical College during January 2004 to December 2013 were collected and analyzed.Results From January 2004 to December 2013,the total mortality rate of hospitalized children was 0.24％ (531/219 429 cases).The mortality rate of 0-28 d was highest (0.77％).The average economic cost of dead cases was 3.4 times compared with other hospitalized cases,three diseases with highest treatment expenses were congenital malformation,deformations and chromosomal abnormalities ; as well as the respiratory system diseases and tumor.Median hospital stay of the dead cases was 5.8 days.The top five causes of death were certain conditions originating in the perinatal period,such as congenital malformation,deformations and chromosomal abnormalities ; injury,poisoning and certain other consequences of external causes;certain infectious and parasitic diseases;diseases of the respiratory system.From January 2004 to December 2013,the mortality rate declined from 0.34％ to 0.12％ (x2 =67.138,P ＜ 0.001),and it was in a descending order.Conclusions Related departments of government should pay more attention to perinatal care,promote the congenital diseases screening,prevent respiratory infections,improve the level of emergency room,reduce poisoning and traffic accidents.And different measures should be taken according to the different characteristics of the death causes of different age groups.
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Objective:To investigate the effects of the anti-TLR2 antibody blocking TLR2 signaling pathway on inflammatory response in Staphylococcus aureus pneumonia murine models.Methods: Sixty C57BL/6J mice were divided randomly into normal control,SA pneumonia,and anti-TLR2 antibody group,killed 3 and 8 days after inoculation respectively.Normal control mice inoculated sterile PBS intranasally ,SA pneumonia mice inoculated SA ,anti-TLR2 antibody group of mice injected with anti-TLR2 antibody by tail vein and then inoculated SA intranasally.At the predetermined point , the colony-forming units ( CFU ) of bacteria were higher , leukocytes and neutrophil percentage were counted in bronchoalveolar lavage fluid ( BALF ) , the concentrations of KC and IL-10 in BALF and serum were assayed by ELISA ,changes in pulmonary histopathology were observed with HE staining and TLR 2 expression was detected by immunohistochemical.Results:3 days after intranasal inoculation ,the concentrations of KC and IL-10 in BALF and serum was increased in SA pneumonia mice , pulmonary histopathology changes significantly in HE staining.Compared with SA pneumonia mice,the CFU of bacteria were higher,leukocytes count and neutrophil percentage ,the concentrations of KC in BALF and serum,as well as HE pathological scores were reduced significantly in anti-TLR2 antibody group mice ,while no significant difference in IL-10.8 days after intranasal inoculation , HE pathological scores of anti-TLR2 antibody group mice were significantly lower than SA pneumonia group mice ,the CFU of bacteria in BALF were not statistically different between those two groups.Conclusion:Anti-TLR2 antibody attenuates the production of inflammatory mediators and inflammatory cell infiltration in SA pneumonia mice .
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Objective To study the disseminated Staphylococcus aureus infection (DSAI) in children. Method Clinical features, treatment and prognosis data of 14 children with DSAI admitted to Yuying Children’s Hospital Afifliated to Wenzhou Medical University from January 2006 to December 2013 was retrospectively reviewed. Results 14 children with DSAI occurred in community, median age:15m (range 6d–13y);50%male (7 cases). All patients presented with fever. Addition to fever, the ifrst symptom was skin and soft tissue infections (SSTIs,6 cases) as well as limb and/or joint pain (5 cases). Among children with DSAI, white blood cell count and C-reactive protein values increased signiifcantly. Pyogenic infection site were skin and soft tissue in 12 cases (85.7%), pulmonary (12 cases), bone (4 cases), joint (3 cases), central nervous system (3 case), and pericardium (1 case). SSTIs concurrent with pulmonary infection was found in 10 cases (71.4%). Incision and drainage of skin and soft tissue abscesses were performed in 9 cases, joint debridement and vacuum sealing drainage (VSD) in 3 cases, osteomyelitis debridement and VSD in 3 cases, and closed chest drainage in 3 cases. All cases received vancomycin and/or linezolid treatment, 5 cases supplemented by rifampicin, and intravenous immune globulin therapy was administered in 11 cases. Clinical manifestations were cured or improved in 12 cases (85.7%). Conclusions Clinical diagnosis of DSAI in children needs to be vigilant. SSTIs, bone and joint infections were major precipitating factors. Intravenous immune globulin therapy was supplemented to the application of antibiotics, which might get better clinical outcomes in children.