effects of ultraviolet B [UV-B] radiation on albino rat cornea and epitheial cells

To evaluate the influence of UV-B radiation on the cornea and epithelial cells of the albino rat. 100 Sprague-Dawely rats were divided in 4 groups with different ages [3, 6, 10, 18 weeks] and control one. Exposed 4 groups were irradiated to UVB with wave length 300nm and intensity of 8 kj/m2. Each group was divided into 2 sub-groups [A and B] according to the time of exposure [15 and 30 minutes]. The control group [C] was divided into 5 rats for each group. One week after exposure cornea was removed to evaluate the histopathological changes. Groups were compared by evaluating the difference between exposed and non-exposed cornea. Exposure to 15 minutes UV-B showed degenerative changes in all ages and increased with time of exposure. Epithelial hyperplasia was 25% in 6 weeks group exposed to 15 minutes of UV-B and 33.3% with 30 minutes of exposure. In 18 weeks group hyperplasia was 25% after 30 minutes of exposure. Epithelial thinning was detected only in 3 and 18 weeks groups after exposure to 30 minutes of UV-B by 33.3%. Epithelial erosion was 16.6% in 10 weeks group after 30 minutes of exposure. Stromal blood vessels were detected in exposed groups to 30 minutes of radiation in 6, 10 weeks by 16.6% and 41.6%. Stromal edema was detected in all ages exposed to 15 and 30 minutes of UV-B. Exposure to UV-B comeal epithelial and stromal changes which increase with time of exposure

effects of ultraviolet B [UV-B] radiation on the lens in albino rats in different ages and times of exposure

To investigate the effects of UVB radiation on the crystalline lens of the rat lens. 100 Sprague-Dawely rats were divided in 4 groups with different ages [3, 6, 10 and 18 weeks] and control one [20 rats in each group]. Exposed 4 groups were irradiated to UVB with wave length 300nm and intensity of 8kj/m2. Each group was divided into 2 subgroups [A and B] according to the time of exposure [15 and 30 minutes]. The control group [C] was divided into 5 rats for each group. One week after exposure, both lenses were removed for morphological changes and for histopathology. Groups were compared by evaluating the difference between exposed and non-exposed lenses. Early cortical cataract [vacuoles] was not detected in all [B] groups, and appeared in all [A] groups As except E4 group. Cortical cataract appeared in both exposed groups and the increased with age. Equatorial cataract was detected in all ages and the incidence increased in rats of [A] groups. Sutural cataract was more in young groups and the incidence increased with time of exposure to UV-B it was not detected in E4 group. Early nuclear and nuclear cataract was more in young ages and increased time of exposure. UV-B has powerful effects on the rat lens and development of different types of cataract, which differs according to the age and time of exposure. The more exposure to UV-B the more the cataract develops