ABSTRACT
Cutaneous round cell tumors have been classified as mast cell tumor [MCT], histiocytoma [HCT], lymphosarcoma, undifferentiated round cell tumors and occasionally rhabdomyosarcoma in veterinary medicine. An adult cock [Gallus domesticus] showing a large solitary integument mass raised on dorso bilateral of cervical part, extending to intercapsular and cranial mid part of the back was referred to the birds clinic of the faculty of veterinary medicine at the university of Tehran. Microscopic examination revealed sheeted cells with large, round to oval nuclei with each one containing one or more prominent nucleoli with scant cytoplasm. The myofibrils of the neck were degenerated by aggressive tumor cells. The condition was differentiated from other round cell tumors by electron microscope, histochemical staining, as well as the application of a large panel of antibodies. Polymerase chain reaction failed to confirm the involvement of both Marek's disease virus and avian leukosis virus subgroup-J. It was concluded that the tumor cells were consistent with both B-cell lymphocytes and histiocytes that unusually covered the entire dermal layer of dorsal neck skin. This unusual cutaneous lymphoma was named as lymphoblastic histiocytoma
ABSTRACT
E-cadherin is a calcium-dependent molecule that contributes to intercellular adhesion. Its proper functioning is important in the maintenance of epithelial structure and integrity. E-cadherin serves as a very important tumor suppressor. In this study, we aim to determine the frequency of E-cadherin expression aberrancy and its relationship to the biological behavior of gastric adenocarcinoma. A total of 52 patients with gastric cancer who underwent gastrectomies in Imam Khomeini Hospital were investigated in a cross-sectional study. Their tissues were stained by immunohistochemistry methods to investigate the expression of E-cadherin. Patients' information including age, gender, Helicobacter pylori infection, lesion location, adenocarcinoma subtype, metastasis, differentiation and regional lymph node involvement, depth of invasion and staging were collected and compared. Age, gender, Helicobacter pylori infection, lesion location, regional lymph node involvement, metastasis, depth of invasion, differentiation and staging did not have a statistically significant relationship with abnormal E-cadherin expression. Abnormal E-cadherin expression was significantly higher in the diffuse sub-type as compared with the intestinal type [90.9% vs. 48.8%, p = 0.016]. The present study assessed the frequency and relationship between abnormal E-cadherin expression and certain biological variables of tumor behavior in Iranian patients with gastric adenocarcinoma. A significant correlation existed only between diffuse sub-typing and reduced E-cadherin expression
ABSTRACT
To verify the myofibroblastic origin of spindle cells by immunohistochemistry in idiopathic inflammatory pseudotumor [IPT] of the orbit. We reviewed a series of 32 orbital IPTs for expression smooth muscle actin [SMA], Vimentin, Desmin and anaplastic lymphokinase [ALK]. The study was performed on histologic samples of 33 patients with a diagnosis of orbital IPT. The lesions expressed SMA in 51.7%, Vimentin in 72.0% and Desmin in 17.2%. All lesions were negative for ALK. Reactivity for SMA in spindle cells may indicate their myofibroblastic origin. The absence of ALK expression in all cases strongly suggests that these lesions, although histologically similar, are biologically distinct from their soft tissue counterparts. IPTs which are negative for ALK may be characterized by one or more chromosomal aberration involving regions other than 2p23
ABSTRACT
Oral lichen planus [OLP] is a chronic immunologic disease. The etiology of OLP is unknown, viral antigens [for example Epstein-Bar virus] have been proposed as etiologic agents. OLP may get transformation to malignancy so research on the presence of EBV in OLP lesions seems to be necessary. The aim of this study was to ascertain if EBV acted as etiologic factor in pathogenesis of OLP. Tissue specimens of 22 patients with clinical diagnosis and histopathological confirmation of OLP were used as case group. And that of 22 persons without OLP served as control group. Polymerase Chain Reaction [PCR] method was used. Each sample was tested twice. All biopsy specimens from patients and controls were negative for EBV presence. In spite of the fact that the presence of EBV in OLP in these two small groups of Iranian population was not confirmed with PCR method, but due to different ideas and reports in this field, proving or disproving of presence or etiological role of EBV in OLP is continuously a question and needs to be examined in further studies
Subject(s)
Humans , Male , Female , Epstein-Barr Virus Infections , Polymerase Chain Reaction , Biopsy , Epstein-Barr Virus Nuclear AntigensABSTRACT
Background: Wilm's tumor is the most frequent primary renal neoplasma in pediatric age group. Classically it is composed of three histologic parts: Blastemal, Epithelial and stromal. Different factors are implicated as prognostic determinants. Nowadays special attention is paid to proliferation markers for determining the biologic behavior of tumors. In this study we tried to ascertain the proliferative index of 22 cases of Wilm's tumor in our center who have had rather good follow up [at least two years]
Materials and Methods: After reviewing the H and E slides, we stained sections with PCNA and ki67 and scanned them by image cytomertry. Then the proliferative indices for each histological part was determined
Results: We resuted that proliferative indices of blastemal and epithelial parts have significant [P< 0.0002] difference [increment] from that of stromal part. Also the patients were divided into those with recurrence [within two ys of primary surgery] and recurrence. The profileration indices of PCNA for those recurring tumors was significantly higher [PCNA= 22.3%] [P= 0.0015]
Conclusion: Finally we concluded that using proliferative markers in Wilm's tumor is useful as an effective prognostic factor