Role of stabilizers in preparation of a potent sheep pox vaccine

Production of live attenuated sheep pox vaccine sustained the elevated temperatures during freeze-drying, transportation storage and vaccination in unequipped tropical and subtropical zones of the world, is highly recommended. For this reason, eight stabilizer formulas were individually used for preparation of eight sheep pox vaccines, which were lyophilized and then titrated and accordingly four vaccine formulas were eventually selected that should be tested for thermoprotectivity to select the best stabilized vaccine. These selected vaccines were tested for sterility; potency [vaccination and challenge] and safety in susceptible sheep. The collected blood sera were subject to serological examination for estimating the antibody response by ELISA. The results proved transcendence of sheep pox vaccines stabilized with 10% trehalose alone or in combination with 5% lactalbumin hydrolyste in the thermoprotectivey, thereby improvement vaccination efficacy

Preparation and evaluation of killed NDV vaccine using new oil adjuvant

Preparation of inactivated oil Newcastle disease virus [NDV] vaccine using different new oil adjuvant Montonide ISA 70 VG, ISA 763A VG and ISA 775 as oil adjuvants in comparison with paraffin oil was carried out. The prepared vaccines with Montanide ISA 70 VG, 763 A VG and 775 VG induced high antibody titers than that induced by vaccines prepared with paraffin oil when determined by serological tests [HI and SNT]. The highest titer observed in prepared vaccine by ISA 70 VG oil adjuvant after 3 weeks post vaccination and remained high till 12 weeks post vaccination and has long duration of immunity reached to 12 month with a protective antibody titer and protection against challenge while prepared vaccine by Montonide ISA 763 A VG oil adjuvant reached 10 month with Montanide ISA 775 reached to 9 months, but in paraffin protective antibody titer reached only till 5 month

Construction of a recombinant baculovirus expressing the major capsid protein [VP6] of bovine rotavirus

The present study reports the expression of VP6, the major inner capsid protein of bovine rotavirus Nebraska calf diarrhea virus [NCDV] strain in a baculovirus expression system. The full-length DNA copies of RNA segment 6 [coding for VP6 protein] of NCDV were inserted into a baculovirus expression vector. A recombinant baculovirus carrying the VP6 gene was constructed through homologous recombination between the baculovirus recombinant plasmid carrying the VP6 gene and Autographa californica nuclear polyhedrosis virus [AcNPV] under the control of the polyhedrin promotor. Infection of Spodoptera frugiperda [Sf9] cells with the recombinant baculovirus expressing VP6 protein revealed a high-level of expression when tested by immunoflurescence and solid phase ELISA tests using BRV-specific polyclonal antibodies. The VP6 expressed protein was detected in Coomassie blue stained SDS-PAGE and produced a detectable band in Western blot assay. The high degree of reactivity with BRV-specific polyclonal antibodies confirmed that the antigenic determinants of the expressed protein were unaltered. The use of the in vitro expressed VP6 protein in the field diagnosis and vaccine development to control rotavirus infection is of considerable intere

Direct immunofluorescence and immunoperoxidase techniques to study the development of rinderpest virus in vero cells and their utility to detect antibodies to rinderpest virus in vaccinated calves

The development of Rinderpest virus [RPV] in Vero cells was studied using direct immunofluorescence [IF] and direct immunoperoxidase [IP] techniques 6, 12, 18, 24, 36, 48, and 72 hours post inoculation of Vero cells with Kabete 0 strain of RPV. Coverslides were fixed and examined with both IF and IP techniques. RPV antigen could be detected 6 hours post inoculation by the direct IF test, while it could be firstly detected 12 hours post inoculation by the direct IP test. Syncytia were observed 48 hours post inoculation. The application of this study was carried to improve the traditional neutralization test. The detection of residual virus activity in cell cultures after neutralizing procedure was done by neutralizing immunofluorescence [NIF] test and by neutralizing peroxidase linked antibody [NPLA] test. Serum samples were collected from calves immunized with the locally prepared TCRR-vaccine and were tested with the abovementioned techniques for detection of neutralizing antibodies to RPV. Statistically there was no significant difference between the two techniques, while NPLA is easier in its application, needs less material and can be done on a large number of sera at a time. Thirteen out of 15 calves had the protective level of antibodies to Rinderpest virus after vaccination with the locally produced TCRV

Preparation of a recent tissue culture antirabies vaccine: growth kinetiks and antigenic properties

In this study, trials were done to propagate SAD rabies virus vaccinal strain on BHK cell line, the virus output after its refreshing reached up to 105/0.1 ml. TCID50, accompanied by marked C.P.E. at the 36th hr p.i. By comparing the growth curve on T.C. with that in mice, it was proved that the highest virus yield happened within the 36th and 60th hr p.i. by 105 TCID50/ml and 107 to 107 MLD50/0.03 ml, respectively. The protection test have the same result, that the highest virus yield was between 36th and 60th hr, which gave a good protection to G. pigs after being challenged with C.V.S