ABSTRACT
Objective:To evaluate the antioxidant activities and phytochemical content of the leaf and root extracts of Rumex crispus using the solvents extraction;methanol extract,ethanol extract,acetone extract (ACE),and water extract.Methods:Total flavonoids content,total phenolic content,and total proanthocyanidin were evaluated using spectrophotometric equivalents of the standards,quercetin,gallic acid and catechin respectively.The antioxidant activities of the plant extracts were determined using ABTS,DPPH,ferric reducing antioxidant power,total antioxidant capacity and nitric oxide scavenging assays.Results:The flavonoids and phenols contents of the extracts were in the range of (19.39 ± 4.08) to (526.23 ± 17.52) mg QE/g and (16.95 ± 12.03) to (240.68 ± 3.50) mg GAE/g,respectively.ACE of the leaf has the highest value of total flavonoids content (526.23 ± 17.52) mg QE/g while ACE of the root has the highest value of total phenolic content (240.68 ± 3.50) mg GAE/g.The highest content of total proanthocyanidin (645.38 ± 1.33) mg CE/g was in ACE of the root.Significant amounts of saponin and alkaloid were also present in the root and leaf extracts.All solvent fractions showed significant antioxidant activities (P ＜ 0.05) with ACE of the root having the highest scavenging value as shown in DPPH,ABTS,total antioxidant capacity,nitric oxide and ferric reducing antioxidant power (IC50 =0.014 mg/mL,＜0.005 mg/mL,0.048 mg/mL,0.067 mg/mL,and 0.075 mg/mL,respectively).Conclusions:In this study,the mean phytochemical content of the root of Rumex crispus is higher than that of the leaf and this may have contributed to its high antioxidant activities.This may also justify the frequent use of the root more than the leaves in traditional medicine for the cure of helminthic infections.