Defeito ósseo de Stafne: relato de dois casos e considerações sobre o diagnóstico / Stafne bone defect: a report of two cases and diagnostic considerations

Defeitos ósseos de Stafne são cavidades ósseas assintomáticas localizadas em mandíbula, frequentemente causadas pela inclusão de tecidos moles. A variante comum desta entidade acomete a região de terceiros molares, abaixo do canal mandibular, sendo geralmente diagnosticada de forma incidental durante exames radiográficos de rotina. A variante em região anterior é incomum e localiza-se nas proximidades dos pré-molares mandibulares. Acredita-se que as glândulas salivares sublinguais estejam implicadas no desenvolvimento desta variante. O objetivo deste relato foi descrever um caso de defeito ósseo de Stafne na região anterior de mandíbula e um caso em mandíbula posterior, com ênfase nos achados clínicos e radiográficos. Cirurgiões dentistas deveriam ter conhecimento desta entidade para evitar biópsias desnecessárias. Na maioria dos casos, acompanhamento clínico-radiográfico constitui a conduta recomendada (AU)

Stafne bone defects are asymptomatic lingual bone depressions of the lower jaw, frequently caused by soft tissue inclusion. The common variant of this entity affects the third molar region, below mandibular canal, and is mostly diagnosed incidentally during routine radiographic examination. The uncommon anterior variant is relatively rare and located in the premolar region of the mandible. Sublingual salivary glands are thought to be responsible for the development of this variant. The aim of this report was to describe a case of Stafne bone defect in the anterior region of mandible and a case in posterior mandible, with emphasis on clinical and radiographic findings. Dental clinicians should be aware of this entity, aiming to avoid unnecessary biopsies. In most cases, clinical and radiographic follow-up is the recommended conduct (AU)

Tooth Calcification in Low-Weight Human Fetuses / Calcificación de Dientes en Fetos Humanos de Bajo Peso

Tooth calcification begins during the intrauterine life and is related to the nutritional status of fetus. The purpose of this study was to evaluate the dental calcification status of 15 low-weight human fetuses aged from the 14th to 28th weeks of intrauterine life through radiographic analyses and von Kossa histochemical technique, which is specific to the detection of calcium and other mineral salts in tissues. After dissection of jaws, the mandibles were submitted to lateral and occlusal radiographies. Three mm thick slide sections of jaws samples were stained by conventional and von Kossa methods. As results, radiographic analysis did not exhibit any suggestive image of tooth mineralization, although rudiments of mandibular ossification could be noted. Hematoxilin and eosin sections revealed tooth germs in advanced phases of odontogenesis, but relative delay in tooth development was observed. The von Kossa staining did not show any positivity to the deposition of mineral salts in tooth germs from maxilla and mandible, supporting our findings related to the delay of dental development that was found in studied sample. Hence, we suggest that there is relation between the fetal low-weight and the calcification and maturation status of teeth.

La calcificación del diente comienza durante la vida intrauterina y se relaciona con el estado nutricional del feto. El propósito de este estudio fue evaluar el estado de calcificación dental de 15 fetos humanos de bajo peso entre las 14 y 28 semanas de vida intrauterina mediante análisis radiográficos y técnica histoquímica von Kossa, específica para la detección de calcio y otras sales minerales en los tejidos. Después de su disección, las mandíbulas fueron sometidas a radiografías laterales y oclusales. Secciones de 3 mm de espesor de las mandibulas fueron teñidas con la técnica Von Kossa. El análisis radiográfico no mostró ninguna imagen sugerente de mineralización dentaria, aunque rudimentos de la osificación mandibular podrían tenerse en cuenta. Las secciones de H-E revelaron la presencia de los gérmenes dentarios en las fases avanzadas de odontogénesis, pero con un retraso relativo en el desarrollo normal. La tinción de von Kossa no mostró positividad a la deposición de sales minerales en gérmenes dentarios, lo que apoya nuestros hallazgos relacionados con el retraso de desarrollo dental que se encontró en la muestra estudiada. Se sugiere que existe relación entre el bajo peso del feto y el estado de calcificación y maduración de los dientes.

CONA and UEA-I ligands during tooth development reveals differences between human and rodents models and shows the beginning of mineral extracelular matrix secretion / Ligandos CONA y UEA-I revelan diferencias entre humanos y en modelos roedores durante el desarrollo dental y muestran el comienzo de la secreción de la matriz mineral extracelular

Many of Glycobyological aspects of human development remain still unclear, mainly in oral science, and this could be observed in a lack of literature with few and old papers about this subject. During tooth histo-morphogenesis changes occur in basement membrane composition, expression of signaling molecules and in localization of cell surface components, where glyco components could be involved. In this sense, this work aimed to analyze the ConA ligands commonly founded in glycoproteins cores and UEA-I ligands since fucolization is a Key event in many physiological and pathological processes. Therefore 15 jaws of human fetuses were ethically obtained, histologicaly processed and then submitted to lectin histochemistry with appropriated controls. The results showed that lectins staining increase their intensity during dental development; ConA only starts to recognize glucose/mannose residues on ectomesechymal cells in the crow phase revealing its ligands when the enamel matrix starts to be secreted. Interestingly, Con A ligands were not founded in the basement membrane of the stratum intermedium of the enamel differing from rodents models. The staining pattern of UEA-I was different, starting to be positive in the ectomesenchyma since the bud stage and shown variable expression in cell type and staining intensity, which appeared be directly proportional to the progress of odontogenesis. Thus, this work shows that Con A and UEA-I exhibit a growing staining directly proporcional to ameloblasts and odontoblasts cytodiferenciation and revels some glycan differences between human odontogenesis and rodents models...

Los aspectos glicobiológicos del desarrollo humano siguen siendo poco investigados, sobre todo en odontología, y esto puede ser observado en la literatura por los escasos y antiguos artículos sobre el tema. Durante la histomorfogénesis del diente se producen cambios en la composición de la membrana basal, en la expresión de moléculas de señalización y en la localización de la superficie celular de los componentes, donde los glico componentes podrían estar involucrados. Este trabajo tuvo como objetivo analizar los ligandos de la ConA ya que glucosa/manosa son comúnmente encontrados en núcleos de glicoproteínas y ligandos de la UEA-I debido a fucolización es un evento clave en muchos procesos fisiológicos y patológicos. Fueron obtenidas 15 mandíbulas de fetos humanos, procesadas y tratadas mediante histoquímica de lectinas con controles apropiados. Los resultados mostraron que la intensidad de tinción de las lectinas aumenta durante el desarrollo del diente. ConA sólo comienza a reconocer residuos de glucosa/manosa en células ectomesénquimales en la fase de corona revelando cuando la matriz de esmalte empieza a ser secretada. Curiosamente, ligandos de la ConA no se encontraron en la membrana basal de la capa intermedia del esmalte, difiriendo de los modelos de roedores. El patrón de tinción de la UEA-I fue diferente, empieza a ser positivo en el ectomesenquima desde la etapa de brotación y muestra variable expresión en el tipo de célula y la intensidad de la tinción, que parecía ser directamente proporcional al progreso de la odontogénesis. Por lo tanto, este trabajo demuestra que la Con A y la UEA-I presentan una coloración que crece directamente proporcional a citodiferenciación de los ameloblastos y odontoblastos, y revela algunas diferencias entre el estándar glicano de odontogénesis humanos y los modelos roedores...

Oral lymphangioma: case report

Introduction: Lymphangioma is a change of lymphatic vessels that frequently affects the head and neck region. Its occurrence at oral cavity is rare and it is most commonly identified at the anterior two-thirds of the tongue. At this location, it is clinically characterized as transparent and generally grouped vesicles, which can be red or purple. The deep lesions appear as nodular masses of variable color and superficial texture. It can be classified according to the size of vessels into three types: capillary, cavernous, and cystic lymphangioma. Several types of treatment have been suggested; and the most commonly used treatments are: surgical excision, application of carbon dioxide laser, cryotherapy using liquid nitrogen, and sclerosing agents. Objective and case report: To describe a case of oral lymphangioma diagnosed in a 17-year-old female patient. The lesion was presented as multiple vesicles of soft consistency with thin epithelial lining and color ranging from translucent to yellow-reddish, involving the soft palate and the left retromolar region. Incisional biopsy confirmed the hypothesis of cavernous lymphangioma. Patient was followed-up for one year without signs of lesion relapse. Conclusion: Through this clinical case report and literature review, this study emphasizes the relevance of the clinical and histopathological features that should be considered to confirm the clinical hypothesis and indicate the proper therapeutic for oral lymphangiomas.

Saliva: métodos atuais para coleta e obtenção da amostra / Saliva: current methods for collection and attainment of the sample

Os métodos para coleta de saliva constituem-se maneiras através das quais podemos mensurar tanto parâmetros biológicos dos componentes da saliva como modificações do volume do fluxo salivar. Existe uma grande variedade de métodos para coleta deste fluido oral, desde os mais simples, como por exemplo uma simples expectoração por parte do próprio indivíduo, o método da drenagem, sucção aberta, sucção fechada, do esfregaço entre outros até a utilização de dispositivos mais sofisticados para coleta como o método salivette, eyespears e o Ultrafiltration Probe. Constitui um procedimento não invasivo, de fácil obtenção e vem sendo cada vez mais utilizado na detecção de antígenos como o citomegalovírus, vírus do herpes simples e anticorpos onde podemos citar o vírus da imunodeficiência humana (HIV), hepatites tipos A, B, C e o helicobacter pylori. Podendo desta forma também nos dar uma idéia da qualidade de saúde da pessoa. Além do potencial uso do fluido oral na detecção de drogas como anfetaminas, opióides e cocaína. O objetivo do presente trabalho é mostrar as inúmeras alternativas para a coleta de saliva, bem como comparar uma técnica a outra, orientando o profissional para a escolha da técnica de coleta que melhor lhe convier.

The methods for saliva collection consist ways through which can measure as many biological parameters of the components of the saliva as modifications of the volume of the flow to salivar. A great variety of methods for collection of this oral fluid exists, since the simplest, as for example a simple spitting on the part of the proper individual, the method of the draining, suction opened, closed suction, swab, among others until the use of sophisticated devices more for collection as the method salivette, eyespears and the Ultrafiltration Probe. It constitutes a not invasive procedure, of easy attainment and comes more being each used time in the antigen detention as citomegalovírus, virus of simple herpes and antibodies where we can cite the virus of the imunodeficiência humana (HIV), hepatites types A, B, C and helicobacter pylori. Giving us an idea of an individual’s health, and the potential oral fluid use detecting amphetamine drugs, opiates and cocaine. The objetive of this article is show innumerable alternatives to saliva collects, as well as comparing one technique to another one guiding the professional for the choice of the collection technique that better to agree to it.