ABSTRACT
BACKGROUND: Rotavirus activity in Korea has been reported beginning in October, peak in November, continuing in winter and ending in spring. But the peak month and the incidence of rotavirus seems to be changed recently. So we investigated the trends of rotavirus activity for the last 10 years in Hanyang University Hospital (HUH). Also latex agglutination test was compared with automated enzyme-linked fluorescent immunoassay for the detection of rotavirus in stool specimens. METHODS: Stool specimens (3,636 from HUH, 1989-1998; 1,171 from Hanyang University Kuri hospital, HUKH, 1996-1998) from pediatric patients with acute diarrhea were tested for rotavirus,. Sixty specimens were tested by latex agglutination test (Sliders Rota-kit 2, bioMerieux Vitek, France) and enzyme-linked fluorescent immunoassay (VIDAS Rotavirus, bioMerieux Vitek, France) according to the instructions from the manufacturer. RESULTS: The annual incidence of rotavirus diarrhea from 1989 to 1998 was 47%, 32%, 33%, 25%, 26%, 24%, 24%, 17%, 17%, 14%, respectively. Positive rate of rotavirus was 25% for the 10year period in HUH, 20% for the recent 3 years in HUKH. Peak month was November (46%) in the first 5 year, but November incidence decreased to 17% in the last 5 year, and the peak moved to January, February, and March (34%, 35%, 33%, respectively). Epidemic period was from October to February during the first 5 year, but from December to April during the last 5 year period. The agreement rate of the two methods was 90% and VIDAS Rotavirus showed significantly higher sensitivity compared to Sliders Rota-kit 2. CONCLUSIONS: The incidence of rotavirus diarrhea decreased gradually for the last 10 years and the peak month of rotavirus activity was changed from November to January, February, and March. The VIDAS Rotavirus was more sensitive than the Sliders Rota-kit 2 for the detection of rotavirus in stool.