Detection of DNA of W. bancrofti in blood samples by QC-PCR-Elisa-based

An internal control was used in a polymerase chain reaction PCR-ELISA-based technique to detect the DNA repeat of the filarial parasite W. bancrofti. The sensitivity of the test could detect as low as one single microfilania added to 200 micro litre of blood. The assay was evaluated on field samples from persons living in areas endemic for filariasis. Examination of night blood of 113 individuals for the presence of microfilania by filtration revealed 44 microfilaria carriers. All microfilaria carriers were positive in the PCR-EL1SA and, in addition, 14 more samples were proven to contain parasite DNA. All the 58 proven cases had circulating filarial antigens in their serum samples. Assuming a sensitivity of PCR-ELISA on night blood of 100%, the sensitivity of night blood filtration was 74% and that of circulating filarial antigens is 100%. The data showed that the described PCR-ELISA method was capable of detecting the filarial infections. Consequently, this method facilitated the identification of the filarial endemic areas and the monitoring of control programs

Detection of anti-fasciola istypes among poatients with fascioliasis before and after treatment with mirazid

Stool examination using modified Kato thick smear method was performed to detect Fasciola eggs and other parasites. Abdominal pain was the major presenting symptom [87.7%], followed by pallor [83.3%] and fever [16.7%]. Anemia and hepatomegaly were recorded in 77.7% of the patients compared with 27.7% with splenomegaly. Abdominal ultrasonography revealed hepatomegaly and common bile duct dilatation in 77.7% of the patients. Moreover, five cases showed diagnostic olympic game rings. All patients had positive IgG4 levels, 55 cases were positive for specific total IgG and IgG1; whereas only 24 cases had positive IgG2 levels. All negative controls showed no cross reactions. On the other hand, ELISA detecting IgG4 showed the highest specificity [95%], followed by IgG2 [85%] and the least specific test was obtained with the detection of IgG [70%] and IgG1 [65%]. One month after treatment, 91.1% of the patients were completely cured and even after another two-month follow up. In completely cured patients, none of anti- Fasciola isotypes was significantly changed. So, the detection of anti- Fasciola isotypes, especially IgG4, is very specific for the accurate diagnosis of human fascioliasis