ABSTRACT
Background: An abnormality in pulse amplitude and frequency of gonadotropin releasing hormone [GnRH] secretion is the most characteristics of polycystic ovarian syndrome [PCOS]. On the other hand, arginine-phenylalanine-amide [RFamide]-related peptide-3 [RFRP3] inhibits the secretion of GnRH in mammalian hypothalamus. The current study performed in order to investigate the expression of RFRP3 mRNA in the dorsomedial hypothalamic nucleus [DMH] after the induction of PCOS in a rat model of constant light exposure, and the possible role of parity on occurrence of PCOS
Materials and Methods: In the experimental study, female nulliparous [n=12] and primiparous [n=12] rats were randomly subdivided into control and PCOS subgroups [n=6]. PCOS were induced by 90 days exposure to constant light. After 90 days, blood, brain, and ovaries were sampled. Serum levels of follicle stimulating hormone [FSH], luteinizing hormone [LH], and testosterone were evaluated. In addition, six adult female ovariectomized rats as a control of real-time polymerase chain reaction [PCR] tests were prepared and in the DMH of all rats, the relative mRNA expression of RFRP3 was assessed
Results: Histological evaluation of ovaries represented the polycystic features. In addition, serum concentrations of testosterone in the PCOS subgroups were more than the controls [P<0.05]. Furthermore, the relative expression of RFRP3 mRNA in PCOS subgroups was lower than the controls [P<0.05]
Conclusion: Constant light model of the PCOS-induced rats decreased the gene expression of RFRP3 in the DMH that suggests the decrease of RFRP3 may reduce its inhibitory effect on GnRH during the PCOS pathogenesis. This effect was stronger in the nulliparous rats than the primiparous
ABSTRACT
Background: Endometrial mesenchymal stem stromal cells [EnMSCs] are critical for uterine function, repair, and regeneration
Objective: This study introduced isolation technique of EnMSCs and compared the characteristics of EnMSCs in mature and immature ewes
Materials and Methods: Endometrial tissue samples from the uterus of 10 ewes were collected from the slaughterhouse. Endometrial cells were isolated from tissue using cold incubation and then chopping and treating was performed with collagenase type I. Isolated cells were cultured in cell culture medium and then attached cells to flasks were harvested as EnMSCs and subcultured. To enumerate the cells, the population doubling time [PDT] was determined and 2.2×104 cells in passage 4 were seeded into 24-well culture plates to compare the growth curves of isolated cells. Reverse transcription polymerase chain reaction [RT-PCR] was performed for detection of CD34 and CD73 markers. The osteogenic and adipogenic potential of isolated cells were determined using differentiation tests
Results: EnMSCs adhered to the flasks and displayed spindle-shape. Based on findings of the cell count and the growth curves, the EnMSCs growth was significantly more prominent in immature ewes in comparison to mature sheep. The PDT of EnMSCs in immature ewes was about 21 hr whereas this time period was two times higher [45 hr] in mature sheep. RT-PCR analyses of EnMSCs were positive for CD73 and negative for CD34. EnMSCs were differentiated into osteoblasts and adipocytes
Conclusion: Based on mesenchymal stem cells characters confirmed in EnMSCs, they can be a candidate for cell therapy and regenerative medicine