ABSTRACT
The minimal inhibitory concentration [MIC] of fresh aqueous garlic extract [AGE] on five strains of Candida Albicans was done using the tube dilution method. The effect of preincubation of Candida strains with the 1 MIC and 1/2 MIC of AGE was investigated on the adhesion of Candida to the buccal epithelial cells [EEC]. Preincubation of EEC with garlic and the adhesion of Candida to this pretreated BEC was also investigated on one of the strains. Adhesion of Candida to BEC was significantly reduced in three strains when the strains were incubated or treated with the 1 MIC of AGE, while it was significantly reduced in one strain only, when the Candida strains were incubated with the 1/2 MIC of AGE. The adhesion of one of the straijis to the pretreated BEC with AGE showed significant reduction
Subject(s)
Mouth Mucosa , Endothelial Cells , Tissue Adhesions , Garlic/drug effectsABSTRACT
500 diarrheal stool specimens were obtained from infants and children suffering from acute gastroenteritis. Isolates of E. coli were identified by conventional methods and they were further subjected to phadebact ETEC-LT coagglutination test, suckling infant mouse assay and hybridization with synthetic enzyme labelled oligonucleotide probe for the detection of heat labile enterotoxigenic E.coli and heat stable enterotoxigenic E.coli. ETEC was detected in 20.8% of specimens. The rate of detection of LT enterotoxin by enzyme labelled LT probe was significantly higher than the phadebact ETEC-LT [McNemnar's X[2] =27.0089]. No significant difference for detection of ST by probe and suckling mouse was found [X[2]=0.05]. The DNA probe hybridization, is recommended for ETEC detection, as an easy rapid technique in minimally equipped laboratories