ABSTRACT
To detect a specific and sensitive marker of bone turnover, this study was conducted on 60 healthy volunteer men classified according to age into three groups: The first group included 20 men in their third decade [with age range of 21-30 years], the second group included 20 men in their fifth decade [with age range of 41-50] and an elderly group included 20 men [age above 60 years]. Blood samples were collected after an overnight fast and serum was separated and kept frozen at -20C until analyzed for bone sialoprotein [BSP], osteocalcin, intact parathyroid hormone [PTH] and calcitonin by radioimmunoassay techniques. Serum BSP may be used as a novel sensitive and specific biochemical marker of bone turnover that may be used alone or in conjunction with osteocalcin in the routine investigations of the elderly for early prediction and follow up of senile osteoporosis
Subject(s)
Biomarkers , Osteocalcin , Sialoglycoproteins , Calcitonin , AgedABSTRACT
This study was conducted on 40 healthy volunteer men in order to find out the relationship between the pineal hormone melatonin, free radicals and age. They were classified according to their ages into two groups, group I comprised 20 men with an age ranging between 41-60 years and group II comprised 20 men with an age ranging between 61-78 years. Morning and evening blood samples were taken from each subject, then melatonin was administered orally in 3 mg single daily dose taken in the evening for six weeks, after which morning blood samples were taken. Serum was separated and analyzed for melatonin and free radicals levels. The results revealed that there was a highly significant decrease in serum melatonin levels in the older age group in comparison with the younger age, while free radicals showed an increase in the older age group compared with the younger age group
ABSTRACT
It is frequently obligatory to expose samples for hormonal assay to different environmental and other processing factors. On the other hand, it is mandatory to identify border-line cases and avoid false results of thyroid dysfunction. These different environmental factors are focused on in this work. Endogenous T3, T4 and TSH were measured by the most convenient ELISA technique in human sera samples exposed to different temperature gradients for different periods. Aliquots of different samples were also exposed to freezing thawing cycles, delayed separation and hemolysis. T3, T4 and TSH assay showed nonsignificant difference in their concentrations when stored for up to 7 days in the refrigerator [4C] and at room temperature. The same results were reported for samples stored frozen at 20C for up to one month. Surprisingly, the 3 hormones were stable also after 5 cycles freezing and thawing, apparent hemolysis and delayed separation for up to 8 hours. These data clarify the degree of stability of the hormones responsible for the diagnosis of thyroid disorders