Attachment of Human Gingival Fibroblasts to Commercially Pure Titanium Surfaces with Different Instruments: A comparative Study in Vitro / 대한치주과학회지

This study examined the human fibroblasts cell attachment to commercially pure titanium surface which had been instrumented by 3 types of periodontal instruments. Commercially pure titanium plates were uniformly scaled using plastic, stainless steel, titanium curette. these all experimental groups 65 undirectional strokes with the designated curettes. Alteration of the surfaces due to instrumentation was evaluated by Form Talysurf(R) and reported as Ra value(mean surface roughness). Then other experimental groups were immersed in a cell suspension of human gingival fibroblasts(1x10(5) cell/ml). After 3 days of culture, cell attachment and morphology was observed by SEM, and attached cell were counted by Hemocytometer. A significant difference in mean Ra value was observed for surface instrumented by metal curette compared to either control surface or surface instrumented by the plastic curette(P<0.01). No stastically significant difference was noted between control surface and those instrumented by the plastic curette. SEM observation showed that cell morphology and attachment to the commercially pure titanium plate was similar appearance on the all experimental groups. Experimental groups instrumented by titanium curette and stainless steel curette were more attached cell number than control group, but experimental group instrumented by plastic curette were similar with control groups(P<0.01). In summary, metal curette produced an significant alteration of the commercially pure titanium surface and more favorable surface topography for cell attachment. Otherwise plastic curette was insignificantly altered the commercially pure titanium surface(P<0.01).

Attachment of Human Gingival Fibroblast to Various Subgingival Restorations: A Comparative Study in Vitro / 대한치주과학회지

When mucoperiosteal flaps are positioned and sutured to desirable position, the wound contains several interface between tissues which differ fundamentally in composition & biological reaction. Thus the C-T surface of the flap will, on one hand, oppose another vascularized surface, and on the other, the avascular dental material for example, when root resoptions, fractured root, endodontic perforation, deep root carious lesions were filled with amalgam, glass ionomer, resin etc. Recently, a number of case report described the successful treatment of a subgingival root lesion with restorative material & free gingival graft, open flap surgery, but more objective research was needed. Most of study on restorative materials were concerned for cytotoxicity not for actual healing event on that materials and its influencing factors such as biocompatibility, surface wettability, surface topography. The aim of this in vitro study was to evaluate the effect of amalgam, resin modified glass ionomer, composite resin per se, and their surface roughness on the growth of human gingival fibroblast. The cells were obtained and placed on culture flask and incubated for 3 days with the prepared test materials. Then count the attached cell number with hemocytometer,(n=12) and 2 samples were examined with SEM about attachment cell morphology. Another 4 samples were evaluated on their surface roughness with Talysurf and average surface roughness value(Ra) were obtained. Statistical difference in attached cell number, roughness value were analyzed using ANOVA. The number of attached cell was as follows, for root dentin specimen 16.7+/-4.41, resin modified glass ionomer 14.0+/-4.15, resin 8.13+/-3.63, amalgam 0.72+/-3.33(x10(3)). Between root dentin and resin-modified glass ionomer, no significant difference was observed, but resin, amalgam showed a significant less cell numbers than for root dentin, resin modified glass ionomer cement. SEM examination expressed many cell surface attachment apparatus in root dentin and resin modified glass ionomer specimens. For resin specimen, cell attachment was observed but exposed less appratus. The average surface roughness value are following results. Dentin specimen 0.6972+/-0.104, resin modified glass ionomer 0.0822+/-0.009, resin 0.0875+/-0.005, amalgam 4.2145+/-0.985(micrometer). Between root dentin, resinmodified glass ionomer, and resin, no significant difference was observed, but amalgam showed a significant more rough surface than other groups. When evlauated the interrelationship between cell attachment and surface roughness, therefore, there was weak reverse correlation.(pearson correlation : -0.593) These results suggest that resin modified glass ionomer have the favorable healing potential when used for subgingival restoration. And for relationship between cell attachment and surface characteristics, further investigations were needed.

The Effect of Various Plaque Control Devices on Roughness and Morphology of Titanium Implant Surface / 대한치주과학회지

The purpose of this study was to compare surface roughness and morphologic changes after use of various plaque control devices to titanium implant surfaces. The study materials were 6 ITI titanium implants(Bonefit(R)) and 5 plaque control devices. 6 implants were divided into 6 different groups and instrumented by each plaque control devices as follows. 1) Group I : untreated control 2) Group II : Titanium curette(Titanium curette(R), 3i) 80 vertical/horizontal strokes 3) Group III : Plastic curette(Implacare(R), Hu-Friedy) 80 vertical/horizontal strokes 4) Group IV : Plastic tip-ultrasonic scaler(Amdent(R), Amdent) 160 seconds 5) Group V : Rotating interdental brush(Identobrush(R), Identoflex) 160 seconds 6) Group VI : Abrasive rubber cup polisher(Zircate(R), Prophy paste, Dentsply) 160 seconds. All specimens were prepared for evaluation by surface roughness tester, optical stereomicroscopy(OM) and scanning electron microscopy(SEM). The Ra and Rt mean values of the tested specimens were 1) Group I (Ra=0.170+/-0.007micrometer, Rt=1.297+/-0.016micrometer) 2) Group II (Ra=0.209+/-0.006micrometer, Rt=1.602+/-0.110micrometer) 3) Group III (Ra=0.179+/-0.001micrometer, Rt=1.429+/-0.055micrometer) 4) Group IV (Ra=0.182+/-0.005micrometer, Rt=1.511+/-0.085micrometer) 5) Group V (Ra=0.301+/-0.008micrometer, Rt=1.882+/-0.131micrometer) 6) Group VI (Ra=0.147+/-0.010micrometer, Rt=1.059+/-0.021micrometer) In Ra values, experimental group II, V, VI were statistically significant different when compared with control. OM and SEM observation showed that experimental group III, IV were minimal changes when compared with control and group VI was smoothest among other experimental groups. The results suggested that plastic curette and plastic tip-ultrasonic scaler were predictable devices to titanium implant surface.