Comparative study of the chronic toxicity of two organotin compounds on albino rats

This study was conducted on 360 adult albino rats of both sexes to evaluatesome of the chronic toxicity of two organotin compounds [TBTO and MBTCL]. Therats were grouped into two control groups orally received the vehicles and twoother groups orally received 1/10 of oral LD50 of TBTO and MBTCL. Monthly forthree months, biochemical and histopathological investigations were done. Tenrats were taken monthly from each group to make their chromosomal patterns. As regards nephrotoxicity, both compounds produced remarkable progressivehistopathological changes with no significant increase in blood urea andcreatinine. The hepatotoxin of both compounds were nearly the same throughthe study [biochemically and histopathologically]. Also, both compounds causeprogressive histopathological changes of almost the same degree in lungs,intestine, brain and heart. Regarding the cytogenetic study, TBTO was foundto be more toxic than MBTCL. This was deduced from the very highlysignificant increase in the number of cells with structural chromosomalanomalies and the total number of structural anomalies in the TBTO treatedgroup as compared with MBTCL treated group. It can be concluded that both TBTOand MBTCL were potentially toxic to the liver, kidneys and lungs. They hadalso mild to moderate toxic effects on intestine, brain and heart. Bothcompounds were highly genotoxic to albino rats. TBTO having more genotoxicpotential. Most of the toxic effects of both compounds were progressive

Light and electron [SEM and TEM] microscope study of the fourth ventricle ependymal lining in rabbit

The ependymal structure of the floor of the fourth ventricle of adult rabbit was investigated using light, scanning and transmission electron microscopy. Eight adult healthy male rabbits were anaesthetized with ether and sacrificed. Small pieces of the floor of fourth ventricle were taken. They were processed for preparation of paraffin sections, stained with Hx and E and examined by L/M. Other minute pieces of them were processed for scanning and transmission electron microscope study. The ependymal cells of the fourth ventricle were seen forming a single row of cuboidal cells with prominent rounded nuclei. Scanning electron microscope showed that the floor of the fourth ventricle exhibited different zones separated by prominent grooves. The ependymal cells exhibited a dense netweek of long cilia. These cilia gradually decreased towards the median sulcus where the ependymal cells were devoid of cilia, but instead they had microvilli on their luminal surfaces. Also pit-like holes appeared in the ependymal lining along the median sulcus, globular supra-ependymal surface projections were noticed. The transmission electron microscope showed that the cells apart from the median sulcus had long and profuse network of interwoven microvilli and cilia over their luminal surface. The cell bodies exhibited supranuelear Golgi complexes, few electron dense granules and multivesiculated bodies. These features suggested a secretory and/or absorptive role