ABSTRACT
An accurate and precise UPLC method was developed for the simultaneous estimation of efavirenz and lamivudine in pharmaceutical dosage forms. The chromatographic analysis was performed on Acquity UPLC BEH Shield RP18 (50 × 3 mm, 1.7 μm) column with mobile phase consisting of 10% acetonitrile in methanol and 10 mM phosphate buffer (pH 4.0) in the gradient mode, at a flow rate of 0.4 mL/min, and eluents monitored at 254 nm. The calibration curves of peak area versus concentration, which was linear from 10-60 μg/mL for efavirenz and 5.0-30 μg/mL for lamivudine, had regression coefficient (r2) greater than 0.999. The method had the requisite accuracy, precision, and robustness for simultaneous determination of efavirenz and lamivudine in tablets. The proposed method is simple, economical, accurate, and precise and could be successfully employed in routine quality control for the simultaneous analysis of efavirenz and lamivudine in pharmaceutical formulations.
ABSTRACT
A simple and rapid high-performance liquid chromatographic (HPLC) method for the determination levocetirizine has been developed. The chromatographic system consisted of a Water 2695 binary gradient pump, Water 2487 dual wavelength absorbance detector, and Empower 2 software. Separation was achieved on the XTerra symmetry C18 column at room temperature. The sample was introduced through an injector valve with a 20 μL sample loop. The results obtained showed a good agreement with the declared content. Recovery values of levocetirizine in tablets were from 99.57-100.48 %. The proposed method is rapid, accurate and selective; it may be used for the quantitative analysis of levocetirizine from raw materials, in bulk drugs and other dosage formulations.