ABSTRACT
BACKGROUND Chagas disease is a public health problem caused by infection with the protozoan Trypanosoma cruzi. There is currently no effective therapy for Chagas disease. Although there is some evidence for the beneficial effect of bone marrow-derived cells in chagasic disease, the mechanisms underlying their effects in the heart are unknown. Reports have suggested that bone marrow cells are recruited to the chagasic heart; however, studies using chimeric mouse models of chagasic cardiomyopathy are rare. OBJECTIVES The aim of this study was to investigate the migration of bone marrow cells to the heart after T. cruzi infection in a model of chagasic disease in chimeric mice. METHODS To obtain chimerical mice, wild-type (WT) C57BL6 mice were exposed to full body irradiation (7 Gy), causing bone marrow ablation. Then, bone marrow cells from green fluorescent protein (GFP)-transgenic mice were infused into the mice. Graft effectiveness was confirmed by flow cytometry. Experimental mice were divided into four groups: (i) infected chimeric (iChim) mice; (ii) infected WT (iWT) mice, both of which received 3 × 104 trypomastigotes of the Brazil strain; (iii) non-infected chimeric (Chim) mice; and (iv) non-infected WT mice. FINDINGS At one-month post-infection, iChim and iWT mice showed first degree atrioventricular block with decreased heart rate and treadmill exercise parameters compared to those in the non-infected groups. MAIN CONCLUSIONS iChim mice showed an increase in parasitaemia, myocarditis, and the presence of amastigote nests in the heart tissue compared to iWT mice. Flow cytometry analysis did not detect haematopoietic progenitor cells in the hearts of infected mice. Furthermore, GFP+ cardiomyocytes were not detected in the tissues of chimeric mice.
Subject(s)
Animals , Female , Mice , Trypanosoma cruzi/physiology , Bone Marrow Cells/physiology , Chagas Cardiomyopathy/parasitology , Bone Marrow Transplantation/methods , Chagas Disease/parasitology , Cell Movement , Animal DiseasesABSTRACT
O exame citologico e um dos mais apropriados metodos de deteccao de lesoes pre-cancerosas e cancer de colo uterino. Porem, uma das criticas mais frequentes a este exame e a alta taxa de falsos negativos que sao atribuidos principalmente a erros de coleta,no escrutinio do esfregaco e na interpretacao do diagnostico. O presente trabalho teve por objetivo avaliar a adequabilidade de amostras de exames citopatologicos realizados no Laboratorio Marques Pereira em Porto Alegre, RS no periodo de janeiro a marco de 2005, de pacientes atendidas pelo Sistema Unico de Saude. Dos 1570 laudos de exames citologicos analisados, 71,40% eram esfregacossatisfatorios, 26,56% satisfatorios, mas limitados por ausencia de celulas glandulares endocervicais, 0,64% satisfatorios, mas limitados pela presenca de sangue, 0,51% satisfatorios, mas limitados por material purulento, 0,38% satisfatorios, mas limitados por areas espessas, 0,19% limitados por dessecamento e 0,32% de material insatisfatorio por lamina danificada ou ausente. Dos 3,24% deexames citologicos que apresentaram algum tipo de alteracoes nas celulas escamosas e glandulares endocervicais, o maior indice foi de LSIL em 1,59% das amostras prevalecendo na faixa etaria dos 21 aos 30 anos. Com referencia a analise microbiologica dos exames citopatologicos, foi verificada uma colonizacao cervico-vaginal predominante de lactobacilos (49,36%).
The cytologic smear is one of the most appropriate methods of pre-cancerous and cervical cancer detection. However,one of the most frequent critics to this examination is the high rate of false negatives that are attributed to the errors of collection mainly, in the scrutiny of smears and in the interpretation of the diagnosis.The purpose of this study was to evaluate the adequateness of citopatological samples, in public health patients, whose examination was realized in Marques Pereira Laboratory in Porto Alegre, RS between january and march of 2005. Of the 1570 exams analyzed, 71.40% were satisfactory smears, 26.56% satisfactory, but limited for absence of endocervical cells, 0.64% satisfactory, but limited forthe presence of blood, 0.51% satisfactory but limited by material with excess of inflammatory cells, 0.38% satisfactory but limited by thick areas, 0.19% limited by desseccate smears and 0.32% of unsatisfactory material for damaged or slyde glass. Of 3.24% of citopathologic exams that presented some alterations in endocervical and squamous cells, the greater index was of LSIL in 1,59% of samplesprevailing in women with 21 to the 30 years. In relation of microbiological analysis of the citopathologic exams, were observed the predominance of Lactobacillus (49,36%).