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Braz. j. microbiol ; 34(1): 72-76, Jan.-Apr. 2003. ilus, graf
Article in English | LILACS | ID: lil-344569

ABSTRACT

An experiment was carried out under greenhouse conditions to observe the distribution of arbuscular mycorrhizal (AM) hyphae in the rhizosphere of papaya and other plants under intercropping system. Special acrylic root boxes with three compartments, separated by nylon mesh screens, were constructed. One outer compartment contained seedlings of papaya (Carica papaya L.) and other contained seedlings of bahiagrass (Paspalum notatum Flügge) or millet (Pennisetum glaucum L. R. Br.). The central compartment was inoculated with an AM fungus (Gigaspora margarita). In another experiment, the outer compartments were treated with 25 percent MeOH eluates of bahiagrass (BRE) and millet (MRE) root extracts. A control box, containning only papaya seedlings was also prepared. Hyphal density, root infection levels and spore numbers were higher in the bahiagrass and millet compartments than in the papaya compartment. Similar results were found for the percentage of AM infection and the number of AM spores. In the compartments treated with BRE and MRE the density of AM hyphae was around 20 percent, and a few spores were found in those compartments. Few AM hyphae and spores were observed in the control compartment.


Subject(s)
Spores, Fungal/growth & development , Spores, Fungal/isolation & purification , Fungi , Hyphae/growth & development , Hyphae/isolation & purification , In Vitro Techniques , Methanol , Carica/growth & development , Methods
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